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BMRB: 19356


19356
4BWH
Solution structure of the chimeric hydrophobin NChi2
Authors
Ren, Q., Sunde, M., Kwan, A.H.
Assembly
MS6
Entity
1. MS6 (polymer, Thiol state: all disulfide bound), 88 monomers, 9024.064 Da Detail

SPAAMERQVP YTPCSGLYGT AQCCATDVLG VADLDCANPP ATLANATHFE STCAAIGQRA RCCKDDVTNT GNSFLIINAA NCQTPAGL


Formula weight
9024.064 Da
Entity Connection
disulfide 4 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS14:SG1:CYS62:SG
2disulfidesing1:CYS23:SG1:CYS53:SG
3disulfidesing1:CYS24:SG1:CYS36:SG
4disulfidesing1:CYS63:SG1:CYS82:SG

Source organism
Neurospora crassa
Exptl. method
solution NMR
Refine. method
ARIA2
Data set
assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 85.7 %, Completeness (bb): 96.1 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All85.7 % (770 of 899)88.8 % (405 of 456)79.2 % (278 of 351)94.6 % (87 of 92)
Backbone96.1 % (496 of 516)96.6 % (170 of 176)95.0 % (245 of 258)98.8 % (81 of 82)
Sidechain75.9 % (353 of 465)83.9 % (235 of 280)64.0 % (112 of 175)60.0 % (6 of 10)
Aromatic57.5 % (23 of 40)95.0 % (19 of 20)20.0 % (4 of 20)
Methyl76.0 % (76 of 100)92.0 % (46 of 50)60.0 % (30 of 50)

1. Nchi2

SPAAMERQVP YTPCSGLYGT AQCCATDVLG VADLDCANPP ATLANATHFE STCAAIGQRA RCCKDDVTNT GNSFLIINAA NCQTPAGL

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 318.000 K, pH 2.500, Details unlabeled, 497 uM in 50 mM glycine, pH 2.5,


#NameIsotope labelingTypeConcentration
1Nchi2natural abundance0.50 mM
2Glycinenatural abundance50.00 mM
3DSSnatural abundance0.05 mM

LACS Plot; CA
Referencing offset: -0.01 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.01 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.28 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 4BWH, Strand ID: A Detail

Heteronucl. T1
80 T1 values in 1 lists
Coherence Sz, Field strength (1H) 600.1299 MHz, Pressure 1.000 atm, Temperature 318.000 K, pH 2.500 Detail
Heteronucl. T2
80 T2 values in 1 lists
Coherence S(+,-), Field strength (1H) 600.1301 MHz, Pressure 1.000 atm, Temperature 318.000 K, pH 2.500 Detail
Heteronucl. NOE
80 NOE values in 1 lists
Value type peak height, Field strength (1H) 600 MHz, Pressure 1.000 atm, Temperature 318.000 K, pH 2.500 Detail
Release date
2013-07-09
Citation
Solution structure and interface-driven self-assembly of NC2, a new member of the Class II hydrophobin proteins
Ren, Q., Kwan, A.H., Sunde, M.
Proteins (2014), 82, 990-1003, PubMed 24218020 , DOI 10.1002/prot.24473 ,
Related entities 1. MS6, : 1 : 3 entities Detail
More details for 4 related entities
Experiments performed 5 experiments Detail
nullKeywords AMYLOID, CELL ADHESION, CHIMERA, INTERFACE, SELF-ASSEMBLY, hydrophobin