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BMRB: 19363

2MAK

Solution structure of the STIM1 CC1-CC2 homodimer in complex with two Orai1 C-terminal domains.

Authors

Stathopulos, P.B., Ikura, M.

Assembly

STIM1 CC1-CC2 homodimer in complex with two Orai1 C-terminal domains

Entity

1. STIM1 CC1-CC2 (polymer, Thiol state: not present), 82 monomers, 9683.832 × 2 Da Detail

GSHMASSRQK YAEEELEQVR EALRKAEKEL ESHSSWYAPE ALQKWLQLTH EVEVQYYNIK KQNAEKQLLV AKEGAEKIKK KR

2. Orai1 (polymer, Thiol state: not present), 23 monomers, 2650.770 × 2 Da Detail

GSELNELAEF ARLQDQLDHR GDH

Total weight

24669.203 Da

Max. entity weight

9683.832 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 81.0 %, Completeness: 73.7 %, Completeness (bb): 76.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.7 % (941 of 1277)	73.0 % (495 of 678)	75.7 % (364 of 481)	69.5 % (82 of 118)
Backbone	76.8 % (482 of 628)	75.1 % (160 of 213)	77.8 % (242 of 311)	76.9 % (80 of 104)
Sidechain	72.0 % (540 of 750)	72.0 % (335 of 465)	74.9 % (203 of 271)	14.3 % (2 of 14)
Aromatic	44.2 % (38 of 86)	44.2 % (19 of 43)	41.5 % (17 of 41)	100.0 % (2 of 2)
Methyl	81.3 % (78 of 96)	83.3 % (40 of 48)	79.2 % (38 of 48)

1. STIM1 CC1-CC2

GSHMASSRQK YAEEELEQVR EALRKAEKEL ESHSSWYAPE ALQKWLQLTH EVEVQYYNIK KQNAEKQLLV AKEGAEKIKK KR

2. Orai1

GSELNELAEF ARLQDQLDHR GDH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	STIM1_CC1-CC2	[U-100% 15N]	0.25 mM
2	Orai1	natural abundance	3.0 mM
3	bis-TRIS	natural abundance	20 mM
4	TFE	[U-99% 2H]	0.175 v/v
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
13	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
14	Orai1	natural abundance	3.0 mM
15	bis-TRIS	natural abundance	20 mM
16	TFE	[U-99% 2H]	0.175 v/v
17	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
18	STIM1_CC1-CC2	natural abundance	2.5 mM
19	Orai1	[U-99% 15N]	0.25 mM
20	bis-TRIS	natural abundance	20 mM
21	TFE	[U-99% 2H]	0.175 v/v
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
24	STIM1_CC1-CC2	natural abundance	2.5 mM
25	Orai1	[U-99% 13C; U-99% 15N]	0.25 mM
26	bis-TRIS	natural abundance	20 mM
27	TFE	[U-99% 2H]	0.175 v/v
28	D2O	natural abundance	100 %

Chem. Shift Complete2

Sequence coverage: 39.0 %, Completeness: 47.7 %, Completeness (bb): 51.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.7 % (1218 of 2554)	47.6 % (645 of 1356)	46.6 % (448 of 962)	53.0 % (125 of 236)
Backbone	51.8 % (651 of 1256)	53.3 % (227 of 426)	48.9 % (304 of 622)	57.7 % (120 of 208)
Sidechain	44.9 % (673 of 1500)	44.9 % (418 of 930)	46.1 % (250 of 542)	17.9 % (5 of 28)
Aromatic	22.1 % (38 of 172)	22.1 % (19 of 86)	20.7 % (17 of 82)	50.0 % (2 of 4)
Methyl	52.1 % (100 of 192)	54.2 % (52 of 96)	50.0 % (48 of 96)

1. STIM1 CC1-CC2

GSHMASSRQK YAEEELEQVR EALRKAEKEL ESHSSWYAPE ALQKWLQLTH EVEVQYYNIK KQNAEKQLLV AKEGAEKIKK KR

2. Orai1

GSELNELAEF ARLQDQLDHR GDH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	STIM1_CC1-CC2	[U-100% 15N]	0.25 mM
2	Orai1	natural abundance	3.0 mM
3	bis-TRIS	natural abundance	20 mM
4	TFE	[U-99% 2H]	0.175 v/v
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
13	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
14	Orai1	natural abundance	3.0 mM
15	bis-TRIS	natural abundance	20 mM
16	TFE	[U-99% 2H]	0.175 v/v
17	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
18	STIM1_CC1-CC2	natural abundance	2.5 mM
19	Orai1	[U-99% 15N]	0.25 mM
20	bis-TRIS	natural abundance	20 mM
21	TFE	[U-99% 2H]	0.175 v/v
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
24	STIM1_CC1-CC2	natural abundance	2.5 mM
25	Orai1	[U-99% 13C; U-99% 15N]	0.25 mM
26	bis-TRIS	natural abundance	20 mM
27	TFE	[U-99% 2H]	0.175 v/v
28	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MAK, Strand ID: A, B, C, D Detail

Release date

2014-01-12

Citation

STIM1/Orai1 coiled-coil interplay in the regulation of store-operated calcium entry
Stathopulos, P.B., Schindl, R., Fahrner, M., Zheng, L., Gasmi-Seabrook, G., Muik, M., Romanin, C., Ikura, M.
Nat. Commun. (2013), 4, 2963-2963, PubMed 24351972 , DOI 10.1038/ncomms3963 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 19362 released on 2014-01-12
    Title Solution Structure of the STIM1 CC1-CC2 homodimer.
  Swiss-Prot: Q96D31 released on 2006-05-16
    Title CRCM1_HUMAN Entity Calcium release-activated calcium channel protein 1
  Swiss-Prot: Q13586 released on 2001-11-02
    Title STIM1_HUMAN Entity Stromal interaction molecule 1

Related entities 1. STIM1 CC1-CC2, : 1 : 1 : 24 entities Detail

Related entities 2. Orai1, : 1 : 7 : 3 entities Detail

Interaction partners 1. STIM1 CC1-CC2, : 31 : 1 interactors Detail

More details for 32 interactors identified by 15 citations

Interaction partners 2. Orai1, : 2 : 1 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	STIM1_CC1-CC2	[U-100% 15N]	0.25 mM
2	Orai1	natural abundance	3.0 mM
3	bis-TRIS	natural abundance	20 mM
4	TFE	[U-99% 2H]	0.175 v/v
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D C(CO)NH TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D H(CCO)NH TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
8	Orai1	natural abundance	3.0 mM
9	bis-TRIS	natural abundance	20 mM
10	TFE	[U-99% 2H]	0.175 v/v
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	STIM1_CC1-CC2	[U-100% 15N]	0.25 mM
2	Orai1	natural abundance	3.0 mM
3	bis-TRIS	natural abundance	20 mM
4	TFE	[U-99% 2H]	0.175 v/v
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
13	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
14	Orai1	natural abundance	3.0 mM
15	bis-TRIS	natural abundance	20 mM
16	TFE	[U-99% 2H]	0.175 v/v
17	D2O	natural abundance	100 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
13	STIM1_CC1-CC2	[U-99% 13C; U-99% 15N]	0.25 mM
14	Orai1	natural abundance	3.0 mM
15	bis-TRIS	natural abundance	20 mM
16	TFE	[U-99% 2H]	0.175 v/v
17	D2O	natural abundance	100 %

10 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
18	STIM1_CC1-CC2	natural abundance	2.5 mM
19	Orai1	[U-99% 15N]	0.25 mM
20	bis-TRIS	natural abundance	20 mM
21	TFE	[U-99% 2H]	0.175 v/v
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

11 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
24	STIM1_CC1-CC2	natural abundance	2.5 mM
25	Orai1	[U-99% 13C; U-99% 15N]	0.25 mM
26	bis-TRIS	natural abundance	20 mM
27	TFE	[U-99% 2H]	0.175 v/v
28	D2O	natural abundance	100 %

12 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
18	STIM1_CC1-CC2	natural abundance	2.5 mM
19	Orai1	[U-99% 15N]	0.25 mM
20	bis-TRIS	natural abundance	20 mM
21	TFE	[U-99% 2H]	0.175 v/v
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

13 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5

#	Name	Isotope labeling	Concentration
24	STIM1_CC1-CC2	natural abundance	2.5 mM
25	Orai1	[U-99% 13C; U-99% 15N]	0.25 mM
26	bis-TRIS	natural abundance	20 mM
27	TFE	[U-99% 2H]	0.175 v/v
28	D2O	natural abundance	100 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19363_2mak.nef
Input source #2: Coordindates	2mak.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

------------320-------330-------340-------350-------360-------370-------380-------
GSHMASSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMASSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKR
--------10--------20--------30--------40--------50--------60--------70--------80--

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-0------280-------290--
GSELNELAEFARLQDQLDHRGDH
|||||||||||||||||||||||
GSELNELAEFARLQDQLDHRGDH
--------10--------20---

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

------------320-------330-------340-------350-------360-------370-------380-------
GSHMASSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMASSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKR
--------10--------20--------30--------40--------50--------60--------70--------80--

Entity_assembly_ID (NMR): D vs Auth_asym_ID (model): D

-0------280-------290--
GSELNELAEFARLQDQLDHRGDH
|||||||||||||||||||||||
GSELNELAEFARLQDQLDHRGDH
--------10--------20---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Sequence coverage (%)
A	A	82	100.0
B	B	23	100.0
C	C	82	100.0
D	D	23	100.0

Content subtype: combined_19363_2mak.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	932		100.0 (chain: A, length: 82)
2	Assigned chemical shifts	assigned_chem_shift_list_2	Warning	216		100.0 (chain: B, length: 23)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	3666 (1)	noe	98.8 (chain: A, length: 82), 100.0 (chain: B, length: 23), 98.8 (chain: C, length: 82), 100.0 (chain: D, length: 23)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	Error	280 (1)	hbond	No information
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	352 (352)	.	90.2 (chain: A, length: 82), 100.0 (chain: B, length: 23), 90.2 (chain: C, length: 82), 100.0 (chain: D, length: 23)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 493
  - ¹³C chemical shifts: 360
  - ¹⁵N chemical shifts: 79
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 125
  - ¹³C chemical shifts: 65
  - ¹⁵N chemical shifts: 26
- Completeness of assignments
  - Entity_assemble_ID: B
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	543	493	90.8
¹³C chemical shifts	384	360	93.8
¹⁵N chemical shifts	96	79	82.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	165	161	97.6
¹³C chemical shifts	164	159	97.0
¹⁵N chemical shifts	81	77	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	378	332	87.8
¹³C chemical shifts	220	201	91.4
¹⁵N chemical shifts	15	2	13.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	39	100.0
¹³C chemical shifts	39	39	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	34	19	55.9
¹³C chemical shifts	32	17	53.1
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	135	123	91.1
¹³C chemical shifts	97	65	67.0
¹⁵N chemical shifts	28	26	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	47	97.9
¹³C chemical shifts	46	23	50.0
¹⁵N chemical shifts	23	23	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	76	87.4
¹³C chemical shifts	51	42	82.4
¹⁵N chemical shifts	5	3	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	10	100.0
¹³C chemical shifts	10	10	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	0	0.0
¹³C chemical shifts	9	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 98.8%
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
  - Entity_assembly_ID: C, Chain length: 82, Sequence coverage: 98.8%
  - Entity_assembly_ID: D, Chain length: 23, Sequence coverage: 100.0%
- Total number of restraints: 3662
- Weight of restraints: 1.0 (3662)
- Potential type of restraints: square-well-parabolic (3662)
- Range of distance target values: 1.28, 2.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
  - Chain_ID: D
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: Error
  - Experiment type: hbond
  - Total number of restraints: 280
  - Weight of restraints: 1.0 (280)
  - Potential type of restraints: square-well-parabolic (280)
  - Range of distance target values: 1.0, 1.5 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
      None
    - Chain_ID: C
    - Chain_ID: D
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 82, Sequence coverage: 90.2%
  - Entity_assembly_ID: B, Chain length: 23, Sequence coverage: 100.0%
  - Entity_assembly_ID: C, Chain length: 82, Sequence coverage: 90.2%
  - Entity_assembly_ID: D, Chain length: 23, Sequence coverage: 100.0%
- Total number of restraints: 352
- Total number of restraints per polymer type: 352
- Weight of restraints: 1.0 (352)
- Potential type of restraints: square-well-parabolic (352)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: B
  - Chain_ID: D

Keywords CC1-CC2, coiled-coil, Orai1, Orai1 C-terminal domain, STIM1

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Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.588 seconds)

BMRB: 19363

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Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

Related entities 1. STIM1 CC1-CC2, : 1 : 1 : 24 entities Detail

Related entities 2. Orai1, : 1 : 7 : 3 entities Detail

Interaction partners 1. STIM1 CC1-CC2, : 31 : 1 interactors Detail

Interaction partners 2. Orai1, : 2 : 1 interactors Detail

Experiments performed 13 experiments Detail

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NMR combined restraints 6 contents Detail