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BMRB: 19376

4BYA

Calmodulin, C-terminal domain, M144H mutant

Authors

Moroz, Y.S., Wu, Y., van Nuland, N.A.J., Korendovych, I.V.

Assembly

Calmodulin, C-terminal domain

Entity

1. Calmodulin, C-terminal domain (polymer, Thiol state: not present), 75 monomers, 8613.305 Da Detail

SLMKDTDSEE EIREAFRVFD KDGNGYISAA ELRHVMTNLG EKLTDEEVDE MIREADIDGD GQVNYEEFVQ HMTAK

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

8653.383 Da

Max. entity weight

8613.305 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.9 % (829 of 847)	98.6 % (434 of 440)	96.6 % (316 of 327)	98.8 % (79 of 80)
Backbone	99.1 % (446 of 450)	99.4 % (154 of 155)	99.1 % (218 of 220)	98.7 % (74 of 75)
Sidechain	97.0 % (453 of 467)	98.2 % (280 of 285)	94.9 % (168 of 177)	100.0 % (5 of 5)
Aromatic	81.5 % (44 of 54)	96.3 % (26 of 27)	66.7 % (18 of 27)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. Calmodulin, C-terminal domain

SLMKDTDSEE EIREAFRVFD KDGNGYISAA ELRHVMTNLG EKLTDEEVDE MIREADIDGD GQVNYEEFVQ HMTAK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin, C-terminal domain	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium azide	natural abundance	0.01 %
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.65 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 4BYA, Strand ID: A Detail

Release date

2014-06-15

Citation

Calmodulin, C-terminal domain, M144H mutant
Moroz, Y.S., Wu, Y., van Nuland, N.A.J., Korendovych, I.V.
Not known

Related entities 1. Calmodulin, C-terminal domain, : 1 : 117 : 440 entities Detail

Interaction partners 1. Calmodulin, C-terminal domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC