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BMRB: 19379

2MAW

NMR structures of the alpha7 nAChR transmembrane domain.

Authors

Bondarenko, V., Mowrey, D., Xu, Y., Tang, P.

Assembly

alpha7 nAChR transmembrane domain

Entity

1. alpha7 nAChR transmembrane domain (polymer, Thiol state: all free), 137 monomers, 14576.95 Da Detail

SNAEEELYYG LNLLIPCVLI SALALLVFLL PADSGEKISL GITVLLSLTV FMLLVAEIMP STSDSSPSIA QYFASTMIIV GLSVVVTVIV LQYHHHDPDG GEGGGEGIDR LCLMAFSVFT IICTIGILMS APNFVEE

Formula weight

14576.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 75.6 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.6 % (1162 of 1538)	71.6 % (554 of 774)	76.1 % (478 of 628)	95.6 % (130 of 136)
Backbone	90.9 % (736 of 810)	93.9 % (262 of 279)	87.3 % (349 of 400)	95.4 % (125 of 131)
Sidechain	62.4 % (533 of 854)	59.0 % (292 of 495)	66.7 % (236 of 354)	100.0 % (5 of 5)
Aromatic	34.6 % (36 of 104)	38.5 % (20 of 52)	30.8 % (16 of 52)
Methyl	79.5 % (178 of 224)	81.3 % (91 of 112)	77.7 % (87 of 112)

1. entity

SNAEEELYYG LNLLIPCVLI SALALLVFLL PADSGEKISL GITVLLSLTV FMLLVAEIMP STSDSSPSIA QYFASTMIIV GLSVVVTVIV LQYHHHDPDG GEGGGEGIDR LCLMAFSVFT IICTIGILMS APNFVEE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7

#	Name	Isotope labeling	Concentration
1	alpha7 nAChR transmembrane domain	[U-100% 13C; U-100% 15N]	0.25 mM
2	NaCl	natural abundacne	10 mM
3	sodium acetate buffer	natural abundacne	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MAW, Strand ID: A Detail

Release date

2013-11-10

Citation

NMR structures of the human α7 nAChR transmembrane domain and associated anesthetic binding sites
Bondarenko, V., Mowrey, D.D., Tillman, T.S., Seyoum, E., Xu, Y., Tang, P.
Biochim. Biophys. Acta (2014), 1838, 1389-1395, PubMed 24384062 , DOI 10.1016/j.bbamem.2013.12.018 , Abstract

Related entities 1. alpha7 nAChR transmembrane domain, : 1 : 4 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC