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BMRB: 19390

2N2J

Solution structure of the EBNA-2 N-terminal Dimerization (END) domain from the Epstein-Barr virus

Authors

Friberg, A., Sattler, M.

Assembly

EBNA-2 N-terminal Dimerization (END) domain

Entity

1. EBNA-2 N-terminal Dimerization (END) domain (polymer, Thiol state: not present), 62 monomers, 6661.432 × 2 Da Detail

GAMEMPTFYL ALHGGQTYHL IVDTDSLGNP SLSVIPSNPY QEQLSDTPLI PLTIFVGENT GV

Total weight

13322.864 Da

Max. entity weight

6661.432 Da

Source organism

Human herpesvirus 4 strain B95-8

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.9 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.9 % (610 of 694)	95.5 % (338 of 354)	78.1 % (217 of 278)	88.7 % (55 of 62)
Backbone	82.2 % (296 of 360)	97.6 % (121 of 124)	67.8 % (122 of 180)	94.6 % (53 of 56)
Sidechain	94.4 % (368 of 390)	94.3 % (217 of 230)	96.8 % (149 of 154)	33.3 % (2 of 6)
Aromatic	96.2 % (50 of 52)	100.0 % (26 of 26)	92.3 % (24 of 26)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. END domain

GAMEMPTFYL ALHGGQTYHL IVDTDSLGNP SLSVIPSNPY QEQLSDTPLI PLTIFVGENT GV

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 323 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	END domain	[U-13C; U-15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	sodium phosphate	natural abundance	20 mM
5	sodium chloride	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.534 ppm	internal	indirect	0.2514495
¹H	water	protons	4.534 ppm	internal	direct	1.0
¹⁵N	water	protons	4.534 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.534 ppm	internal	indirect	0.2514495
¹H	water	protons	4.534 ppm	internal	direct	1.0
¹⁵N	water	protons	4.534 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.534 ppm	internal	indirect	0.2514495
¹H	water	protons	4.534 ppm	internal	direct	1.0
¹⁵N	water	protons	4.534 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2N2J, Strand ID: A, B Detail

Release date

2015-06-07

Citation

The EBNA-2 N-Terminal Transactivation Domain Folds into a Dimeric Structure Required for Target Gene Activation
Friberg, A., Thumann, S., Hennig, J., Zou, P., Ling, P.D., Sattler, M., Kempkes, B.
PLoS Pathog. (2015), 11, e1004910-e1004910, PubMed 26024477 , DOI 10.1371/journal.ppat.1004910 , Abstract

Related entities 1. EBNA-2 N-terminal Dimerization (END) domain, : 1 : 7 entities Detail

Interaction partners 1. EBNA-2 N-terminal Dimerization (END) domain, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC