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Human Bcl10 CARD
Authors
Zheng, C., Bracken, C., Wu, H.
Assembly
CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly
Entity
1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly (polymer), 118 monomers, 13824.75 × 20 Da Detail

GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLK


Total weight
276495.0 Da
Max. entity weight
13824.75 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.3 %, Completeness: 86.9 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.9 % (1249 of 1438)84.6 % (645 of 762)87.9 % (487 of 554)95.9 % (117 of 122)
Backbone96.4 % (677 of 702)93.6 % (221 of 236)98.6 % (346 of 351)95.7 % (110 of 115)
Sidechain80.6 % (686 of 851)80.6 % (424 of 526)80.2 % (255 of 318)100.0 % (7 of 7)
Aromatic26.9 % (14 of 52)53.8 % (14 of 26) 0.0 % (0 of 26)
Methyl98.6 % (140 of 142)98.6 % (70 of 71)98.6 % (70 of 71)

1. Bcl10

GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLK

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Bcl10[U-13C; U-15N]0.3 ~ 0.4 mM
2H2Onatural abundance93 %
3D2Onatural abundance7 %

LACS Plot; CA
Referencing offset: -0.04 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.04 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.07 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.99 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2MB9, Strand ID: A Detail


Release date
2013-10-13
Citation
Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly
Qiao, Q., Yang, C., Zheng, C., Fontan, L., David, L., Yu, X., Bracken, C., Rosen, M., Melnick, A.H., Egelman, E., Wu, H.
Mol. Cell (2013), 51, 766-779, PubMed 24074955 , DOI 10.1016/j.molcel.2013.08.032 ,
Related entities 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 1 : 9 : 4 entities Detail
Interaction partners 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 41 interactors Detail
Experiments performed 1 experiments Detail
NMR combined restraints 4 contents Detail
Keywords Bcl10, CARD, Death domain