BMRBj - BMREntryMaster

Found 1 Document (1.461 seconds)

BMRB: 19392

2MB9

Human Bcl10 CARD

Authors

Zheng, C., Bracken, C., Wu, H.

Assembly

CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly

Entity

1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly (polymer), 118 monomers, 13824.75 × 20 Da Detail

GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLK

Total weight

276495.0 Da

Max. entity weight

13824.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 86.9 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (1249 of 1438)	84.6 % (645 of 762)	87.9 % (487 of 554)	95.9 % (117 of 122)
Backbone	96.4 % (677 of 702)	93.6 % (221 of 236)	98.6 % (346 of 351)	95.7 % (110 of 115)
Sidechain	80.6 % (686 of 851)	80.6 % (424 of 526)	80.2 % (255 of 318)	100.0 % (7 of 7)
Aromatic	26.9 % (14 of 52)	53.8 % (14 of 26)	0.0 % (0 of 26)
Methyl	98.6 % (140 of 142)	98.6 % (70 of 71)	98.6 % (70 of 71)

1. Bcl10

GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLK

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Bcl10	[U-13C; U-15N]	0.3 ~ 0.4 mM
2	H2O	natural abundance	93 %
3	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.99 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MB9, Strand ID: A Detail

Release date

2013-10-13

Citation

Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly
Qiao, Q., Yang, C., Zheng, C., Fontan, L., David, L., Yu, X., Bracken, C., Rosen, M., Melnick, A.H., Egelman, E., Wu, H.
Mol. Cell (2013), 51, 766-779, PubMed 24074955 , DOI 10.1016/j.molcel.2013.08.032 , Abstract

Related entities 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 1 : 9 : 4 entities Detail

Interaction partners 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 41 interactors Detail

More details for 41 interactors identified by 17 citations

Experiments performed 1 experiments Detail

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19392_2mb9.nef
Input source #2: Coordindates	2mb9.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-----------10--------20--------30--------40--------50--------60--------70--------80--------90-------
GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

100-------110-----
KITDEVLKLRNIKLEHLK
||||||||||||||||||
KITDEVLKLRNIKLEHLK
-------110--------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	118	0	0	100.0

Content subtype: combined_19392_2mb9.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1219		97.5 (chain: A, length: 118)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	848 (1)	noe	89.0 (chain: A, length: 118)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	203 (203)	.	89.0 (chain: A, length: 118)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 118, Sequence coverage: 97.5%
- Total number of assignments
  - ¹H chemical shifts: 637
  - ¹³C chemical shifts: 466
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	762	637	83.6
¹³C chemical shifts	554	466	84.1
¹⁵N chemical shifts	133	116	87.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	236	227	96.2
¹³C chemical shifts	236	230	97.5
¹⁵N chemical shifts	115	109	94.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	526	410	77.9
¹³C chemical shifts	318	236	74.2
¹⁵N chemical shifts	18	7	38.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	70	97.2
¹³C chemical shifts	72	66	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	14	53.8
¹³C chemical shifts	26	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 118, Sequence coverage: 89.0%
- Total number of restraints: 848
- Weight of restraints: 1.0 (848)
- Potential type of restraints: square-well-parabolic (848)
- Range of distance target values: 1.85, 3.65 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 118, Sequence coverage: 89.0%
- Total number of restraints: 203
- Total number of restraints per polymer type: 203
- Weight of restraints: 1.0 (203)
- Potential type of restraints: square-well-parabolic (203)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Bcl10, CARD, Death domain

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Found 1 Document (1.461 seconds)

BMRB: 19392

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 1 : 9 : 4 entities Detail

Interaction partners 1. CARMA1/Bcl10/MALT1 Signalosome: Nucleation Induced Filamentous Assembly, : 41 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

NMR combined restraints 4 contents Detail