Human Bcl10 CARD
GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.9 % (1249 of 1438) | 84.6 % (645 of 762) | 87.9 % (487 of 554) | 95.9 % (117 of 122) |
Backbone | 96.4 % (677 of 702) | 93.6 % (221 of 236) | 98.6 % (346 of 351) | 95.7 % (110 of 115) |
Sidechain | 80.6 % (686 of 851) | 80.6 % (424 of 526) | 80.2 % (255 of 318) | 100.0 % (7 of 7) |
Aromatic | 26.9 % (14 of 52) | 53.8 % (14 of 26) | 0.0 % (0 of 26) | |
Methyl | 98.6 % (140 of 142) | 98.6 % (70 of 71) | 98.6 % (70 of 71) |
1. Bcl10
GSHMEPTAPS LTEEDLTEVK KDALENLRVY LCEKIIAERH FDHLRAKKIL SREDTREISC RTSSRKRAGK LLDYLQENPK GLDTLVESIR REKTQNFLIQ KITDEVLKLR NIKLEHLKSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Bcl10 | [U-13C; U-15N] | 0.3 ~ 0.4 mM | |
2 | H2O | natural abundance | 93 % | |
3 | D2O | natural abundance | 7 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Varian Unity - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Bcl10 | [U-13C; U-15N] | 0.3 ~ 0.4 mM | |
2 | H2O | natural abundance | 93 % | |
3 | D2O | natural abundance | 7 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_19392_2mb9.nef |
Input source #2: Coordindates | 2mb9.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
-----------10--------20--------30--------40--------50--------60--------70--------80--------90------- GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 100-------110----- KITDEVLKLRNIKLEHLK |||||||||||||||||| KITDEVLKLRNIKLEHLK -------110--------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 118 | 0 | 0 | 100.0 |
Content subtype: combined_19392_2mb9.nef
Assigned chemical shifts
-----------10--------20--------30--------40--------50--------60--------70--------80--------90------- GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| ..HMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTS.RKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ 100-------110----- KITDEVLKLRNIKLEHLK |||||||||||||||||| KITDEVLKLRNIKLEHLK
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 762 | 637 | 83.6 |
13C chemical shifts | 554 | 466 | 84.1 |
15N chemical shifts | 133 | 116 | 87.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 236 | 227 | 96.2 |
13C chemical shifts | 236 | 230 | 97.5 |
15N chemical shifts | 115 | 109 | 94.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 526 | 410 | 77.9 |
13C chemical shifts | 318 | 236 | 74.2 |
15N chemical shifts | 18 | 7 | 38.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 72 | 70 | 97.2 |
13C chemical shifts | 72 | 66 | 91.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 26 | 14 | 53.8 |
13C chemical shifts | 26 | 0 | 0.0 |
Distance restraints
-----------10--------20--------30--------40--------50--------60--------70--------80--------90------- GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| ............EEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTS.RKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ 100-------110----- KITDEVLKLRNIKLEHLK |||||||||||||||||| KITDEVLKLRNIKLEHLK
Dihedral angle restraints
-----------10--------20--------30--------40--------50--------60--------70--------80--------90------- GSHMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ............EEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTREISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQ -----------10--------20--------30--------40--------50--------60--------70--------80--------90------- 100-------110----- KITDEVLKLRNIKLEHLK ||||||||||||||||| KITDEVLKLRNIKLEHL 100-------110----