BMRB: 19396

Lasiocepsin

Authors

Monincova, L., Budesinsky, M., Cujova, S., Cerovsky, V., Veverka, V.

Assembly

Lasiocepsin

Entity

1. Lasiocepsin (polymer, Thiol state: all disulfide bound), 27 monomers, 2897.763 Da Detail

GLPRKILCAI AKKKGKCKGP LKLVCKC

Formula weight

2897.763 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS25:SG
2	disulfide	sing	1:CYS17:SG	1:CYS27:SG

Source organism

Lasioglossum laticeps

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.9 %, Completeness (bb): 88.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.9 % (287 of 334)	95.6 % (173 of 181)	70.3 % (90 of 128)	96.0 % (24 of 25)
Backbone	88.0 % (139 of 158)	96.4 % (53 of 55)	79.5 % (62 of 78)	96.0 % (24 of 25)
Sidechain	85.5 % (171 of 200)	95.2 % (120 of 126)	68.9 % (51 of 74)
Methyl	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)

1. lasiocepsin

GLPRKILCAI AKKKGKCKGP LKLVCKC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	H2O	natural abundance		90 %
2	D2O	natural abundance		10 %
3	lasiocepsin	natural abundance		4 mM

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Protein Blocks Logo

Calculated from 48 models in PDB: 2MBD, Strand ID: A Detail

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Release date

2014-01-12

Citation

Structural basis for antimicrobial activity of lasiocepsin
Monincova, L., Budinsky, M., Cujova, S., Ceovsky, V., Veverka, V.
Chembiochem. (2014), 15, 301-308, PubMed 24339323 , DOI 10.1002/cbic.201300509 , Abstract

Related entities 1. Lasiocepsin, : 1 : 1 : 3 entities Detail

Experiments performed 4 experiments Detail