BMRB: 19400

The structure of the Box CD enzyme reveals regulation of rRNA methylation

Authors

Lapinaite, A., Simon, B., Skjaerven, L., Rakwalska-bange, M., Gabel, F., Carlomagno, T.

Assembly

The structure of the Box CD enzyme reveals regulation of rRNA methylation

Entity

1. SSR26 (polymer, Thiol state: not present), 72 monomers, 23588.06 × 2 Da Detail

GCGAGCAAUG AUGAGUGAUG GGCGAACUGA GCUCGAAAGA GCAAUGAUGA GUGAUGGGCG AACUGAGCUC GC

2. NOP5-NOP56 RELATED PROTEIN (polymer, Thiol state: all free), 366 monomers, 42138.22 × 4 Da Detail

FISENVRGIY AFDENGNLIE KRYFTDKPEK VLDQLLKGEI TKDLEELLNS LKEKGYDEFV FEHPCLSRRA KELGFSATTE FPNIAGERLR SNPCEFLGEN WFEEYYKVGV ALTRMRIQEQ SGARDKMVIQ AIEALDDVDK VINLLVARLR EWYSLHFPEL DELLPKHPQY VAFVKTVGHR DNINEEVLRC LGLSECKIKK ILEAKEKTMG AWMDQTDIEV VRQLAEEIDR LYQLRKKLED YIDRAMCDVA PNLKALVGAK LAARLISLAG GLRELAMMPS STIQVLGAEK ALFRHLRTGA KPPKHGVIYQ YPAINRSPWW QRGKIARALA GKLAIAARVD YFCGEYIAEE LKKELEARIR EIKEKY

3. 50S RIBOSOMAL PROTEIN L7AE (polymer, Thiol state: all free), 121 monomers, 13178.39 × 4 Da Detail

KPSYVKFEVP KCLAEKALQA VEIARDTGKI RKGTNETTKA VERGCAKLVI IAEDVDPEEI VAHLPPLCEE CEIPYIYVPC KKELGAAACI EVAAASVAII EPGKARDLVE EIAMKVKELM K

4. FIBRILLARIN-LIKE RRNA-TRNA 2'-O-METHYLTRANSFERASE (polymer, Thiol state: not present), 227 monomers, 25761.40 × 4 Da Detail

MVEVKKHKFP GVYVVIDDDG SEKIATKNLV PGQRVYGERV IKWEGEEYRI WNPHRSKLGA AIVNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK IYGIEFSPRV LRELVPIVEE RRNIIPILGD ATKPEEYRAL VTKVDVIFED VAQPTQAKIL IDNAKAYLKR GGYGMIAVKS RSIDVTKEPE QVFKEVEREL SEYFEVIERL NLEPYEKDHA LFVVRKP

5. 5'-R(*UP*CP*GP*CP*CP*CP*AP*UP*CP*AP*CP)-3' (polymer, Thiol state: not present), 11 monomers, 3465.054 × 4 Da Detail

UCGCCCAUCA C

Total weight

385348.38 Da

Max. entity weight

42138.22 Da

Source organism

Pyrococcus furiosus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 13.0 %, Completeness: 5.3 %, Completeness (bb): 4.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
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Polymer type: polyribonucleotide polypeptide(L)

	Total	1H	13C
All	5.3 % (485 of 9092)	5.1 % (265 of 5190)	5.6 % (220 of 3902)
Backbone	4.6 % (206 of 4449)	4.4 % (85 of 1935)	4.8 % (121 of 2514)
Sidechain	6.0 % (321 of 5314)	5.5 % (180 of 3255)	6.8 % (141 of 2059)
Aromatic	0.5 % (4 of 876)	0.4 % (2 of 463)	0.5 % (2 of 413)
Methyl	20.4 % (180 of 884)	21.0 % (93 of 442)	19.7 % (87 of 442)

1. SSR26

GCGAGCAAUG AUGAGUGAUG GGCGAACUGA GCUCGAAAGA GCAAUGAUGA GUGAUGGGCG AACUGAGCUC GC

2. NOP5-NOP56 RELATED PROTEIN

FISENVRGIY AFDENGNLIE KRYFTDKPEK VLDQLLKGEI TKDLEELLNS LKEKGYDEFV FEHPCLSRRA KELGFSATTE FPNIAGERLR SNPCEFLGEN WFEEYYKVGV ALTRMRIQEQ SGARDKMVIQ AIEALDDVDK VINLLVARLR EWYSLHFPEL DELLPKHPQY VAFVKTVGHR DNINEEVLRC LGLSECKIKK ILEAKEKTMG AWMDQTDIEV VRQLAEEIDR LYQLRKKLED YIDRAMCDVA PNLKALVGAK LAARLISLAG GLRELAMMPS STIQVLGAEK ALFRHLRTGA KPPKHGVIYQ YPAINRSPWW QRGKIARALA GKLAIAARVD YFCGEYIAEE LKKELEARIR EIKEKY

3. 50S RIBOSOMAL PROTEIN L7AE

KPSYVKFEVP KCLAEKALQA VEIARDTGKI RKGTNETTKA VERGCAKLVI IAEDVDPEEI VAHLPPLCEE CEIPYIYVPC KKELGAAACI EVAAASVAII EPGKARDLVE EIAMKVKELM K

4. FIBRILLARIN-LIKE RRNA-TRNA 2'-O-METHYLTRANSFERASE

MVEVKKHKFP GVYVVIDDDG SEKIATKNLV PGQRVYGERV IKWEGEEYRI WNPHRSKLGA AIVNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK IYGIEFSPRV LRELVPIVEE RRNIIPILGD ATKPEEYRAL VTKVDVIFED VAQPTQAKIL IDNAKAYLKR GGYGMIAVKS RSIDVTKEPE QVFKEVEREL SEYFEVIERL NLEPYEKDHA LFVVRKP

5. 5'-R(*UP*CP*GP*CP*CP*CP*AP*UP*CP*AP*CP)-3'

UCGCCCAUCA C

Show sample condition

Sample

Solvent system 99% D2O, Pressure 1 atm, Temperature 328.000 K, pH 6.600, Details 0.014-0.03mmol/l

#	Name	Isotope labeling	Type	Concentration
1	SSR26	natural abundance		0.014 ~ 0.03 mM
2	NOP5/NOP56 RELATED PROTEIN	natural abundance		0.014 ~ 0.03 mM
3	50S RIBOSOMAL PROTEIN L7AE	natural abundance		0.014 ~ 0.03 mM
4	FIBRILLARIN-LIKE RRNA/TRNA 2'-O-METHYLTRANSFERASE	[U-13C]		0.014 ~ 0.03 mM
5	5'-R(*UP*CP*GP*CP*CP*CP*AP*UP*CP*AP*CP)-3'	natural abundance		0.014 ~ 0.03 mM

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Release date

2013-10-07

Citation

The structure of the box C/D enzyme reveals regulation of RNA methylation
Lapinaite, A., Simon, B., Skjaerven, L., Rakwalska-Bange, M., Gabel, F., Carlomagno, T.
Nature (2013), 502, 519-523, PubMed 24121435 , DOI 10.1038/nature12581 , Abstract

Related entities 2. NOP5-NOP56 RELATED PROTEIN, : 1 : 4 : 69 entities Detail

Related entities 3. 50S RIBOSOMAL PROTEIN L7AE, : 33 entities Detail

Related entities 4. FIBRILLARIN-LIKE RRNA-TRNA 2'-O-METHYLTRANSFERASE, : 1 : 5 : 1 : 160 entities Detail

Related entities 5. 5'-R(*UP*CP*GP*CP*CP*CP*AP*UP*CP*AP*CP)-3', : 3 entities Detail

Interaction partners 4. FIBRILLARIN-LIKE RRNA-TRNA 2'-O-METHYLTRANSFERASE, : 2 interactors Detail

Experiments performed 1 experiments Detail