BMRB: 19412

K11-linked Diubiquitin average solution structure at pH 6.8, 150 mM NaCl

Authors

Castaneda, C.A., Fushman, D.

Assembly

K11-linked Diubiquitin

Entity

1. K11-linked Diubiquitin (polymer, Thiol state: not present), 152 monomers, 17111.45 × 2 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR GG

Total weight

34222.9 Da

Max. entity weight

17111.45 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

in-house RDC (orientation) and MTSL (distance)

Data set

assigned_chemical_shifts

Chemical Shifts 1

¹H: 69, ¹⁵N: 69 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	2	GLN	H	H	8.868
2	1	2	GLN	N	N	123.029
3	1	3	ILE	H	H	8.284
4	1	3	ILE	N	N	115.106
5	1	4	PHE	H	H	8.571
6	1	4	PHE	N	N	118.718
7	1	5	VAL	H	H	9.278
8	1	5	VAL	N	N	121.612
9	1	6	LYS	H	H	9.032
10	1	6	LYS	N	N	128.105
11	1	7	THR	H	H	8.625
12	1	7	THR	N	N	115.240
13	1	8	LEU	H	H	9.194
14	1	8	LEU	N	N	121.612
15	1	9	THR	H	H	7.618
16	1	9	THR	N	N	105.816
17	1	10	GLY	H	H	7.849
18	1	10	GLY	N	N	109.497
19	1	11	LYS	H	H	7.252
20	1	11	LYS	N	N	121.511
21	1	12	THR	H	H	8.652
22	1	12	THR	N	N	120.259
23	1	13	ILE	H	H	9.519
24	1	13	ILE	N	N	127.689
25	1	14	THR	H	H	8.712
26	1	14	THR	N	N	121.636
27	1	15	LEU	H	H	8.676
28	1	15	LEU	N	N	125.124
29	1	16	GLU	H	H	8.114
30	1	16	GLU	N	N	122.775
31	1	17	VAL	H	H	8.913
32	1	17	VAL	N	N	117.682
33	1	18	GLU	H	H	8.635
34	1	18	GLU	N	N	119.386
35	1	20	SER	H	H	7.010
36	1	20	SER	N	N	103.545
37	1	21	ASP	H	H	8.034
38	1	21	ASP	N	N	124.073
39	1	22	THR	H	H	7.858
40	1	22	THR	N	N	109.123
41	1	23	ILE	H	H	8.508
42	1	23	ILE	N	N	121.422
43	1	25	ASN	H	H	7.909
44	1	25	ASN	N	N	121.486
45	1	26	VAL	H	H	8.100
46	1	26	VAL	N	N	122.416
47	1	27	LYS	H	H	8.540
48	1	27	LYS	N	N	119.120
49	1	28	ALA	H	H	7.964
50	1	28	ALA	N	N	123.624
51	1	29	LYS	H	H	7.849
52	1	29	LYS	N	N	120.481
53	1	30	ILE	H	H	8.271
54	1	30	ILE	N	N	121.521
55	1	31	GLN	H	H	8.547
56	1	31	GLN	N	N	123.679
57	1	32	ASP	H	H	8.031
58	1	32	ASP	N	N	120.006
59	1	33	LYS	H	H	7.421
60	1	33	LYS	N	N	115.673
61	1	34	GLU	H	H	8.725
62	1	34	GLU	N	N	114.226
63	1	35	GLY	H	H	8.498
64	1	35	GLY	N	N	108.975
65	1	39	ASP	H	H	8.521
66	1	39	ASP	N	N	113.761
67	1	40	GLN	H	H	7.798
68	1	40	GLN	N	N	116.978
69	1	41	GLN	H	H	7.472
70	1	41	GLN	N	N	118.137
71	1	42	ARG	H	H	8.476
72	1	42	ARG	N	N	123.155
73	1	43	LEU	H	H	8.759
74	1	43	LEU	N	N	124.507
75	1	44	ILE	H	H	9.108
76	1	44	ILE	N	N	122.610
77	1	45	PHE	H	H	8.824
78	1	45	PHE	N	N	125.092
79	1	46	ALA	H	H	8.986
80	1	46	ALA	N	N	133.179
81	1	47	GLY	H	H	8.117
82	1	47	GLY	N	N	102.661
83	1	48	LYS	H	H	7.936
84	1	48	LYS	N	N	122.016
85	1	49	GLN	H	H	8.642
86	1	49	GLN	N	N	123.222
87	1	50	LEU	H	H	8.531
88	1	50	LEU	N	N	125.699
89	1	51	GLU	H	H	8.390
90	1	51	GLU	N	N	123.318
91	1	52	ASP	H	H	8.157
92	1	52	ASP	N	N	120.611
93	1	54	ARG	H	H	7.434
94	1	54	ARG	N	N	119.537
95	1	55	THR	H	H	8.808
96	1	55	THR	N	N	108.948
97	1	56	LEU	H	H	8.137
98	1	56	LEU	N	N	118.142
99	1	57	SER	H	H	8.441
100	1	57	SER	N	N	113.575
101	1	58	ASP	H	H	7.918
102	1	58	ASP	N	N	124.676
103	1	59	TYR	H	H	7.235
104	1	59	TYR	N	N	115.914
105	1	60	ASN	H	H	8.125
106	1	60	ASN	N	N	115.972
107	1	61	ILE	H	H	7.241
108	1	61	ILE	N	N	119.163
109	1	62	GLN	H	H	7.643
110	1	62	GLN	N	N	125.087
111	1	63	LYS	H	H	8.452
112	1	63	LYS	N	N	120.602
113	1	64	GLU	H	H	9.287
114	1	64	GLU	N	N	115.358
115	1	65	SER	H	H	7.724
116	1	65	SER	N	N	115.187
117	1	66	THR	H	H	8.681
118	1	66	THR	N	N	117.545
119	1	67	LEU	H	H	9.377
120	1	67	LEU	N	N	127.981
121	1	68	HIS	H	H	9.223
122	1	68	HIS	N	N	119.683
123	1	69	LEU	H	H	8.272
124	1	69	LEU	N	N	123.979
125	1	70	VAL	H	H	9.145
126	1	70	VAL	N	N	126.790
127	1	71	LEU	H	H	8.187
128	1	71	LEU	N	N	123.635
129	1	72	ARG	H	H	8.518
130	1	72	ARG	N	N	123.425
131	1	73	LEU	H	H	8.307
132	1	73	LEU	N	N	123.973
133	1	74	ARG	H	H	8.589
134	1	74	ARG	N	N	122.227
135	1	75	GLY	H	H	8.655
136	1	75	GLY	N	N	110.300
137	1	76	GLY	H	H	8.295
138	1	76	GLY	N	N	108.902

Chemical Shifts 2

¹H: 69, ¹⁵N: 69 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
139	1	2	GLN	H	H	8.880
140	1	2	GLN	N	N	123.236
141	1	3	ILE	H	H	8.279
142	1	3	ILE	N	N	115.200
143	1	4	PHE	H	H	8.557
144	1	4	PHE	N	N	118.584
145	1	5	VAL	H	H	9.264
146	1	5	VAL	N	N	121.742
147	1	6	LYS	H	H	8.973
148	1	6	LYS	N	N	127.775
149	1	7	THR	H	H	8.643
150	1	7	THR	N	N	114.483
151	1	8	LEU	H	H	9.243
152	1	8	LEU	N	N	121.566
153	1	9	THR	H	H	7.588
154	1	9	THR	N	N	105.794
155	1	10	GLY	H	H	7.756
156	1	10	GLY	N	N	109.060
157	1	11	LYS	H	H	7.256
158	1	11	LYS	N	N	122.217
159	1	12	THR	H	H	8.518
160	1	12	THR	N	N	119.619
161	1	13	ILE	H	H	9.540
162	1	13	ILE	N	N	127.701
163	1	14	THR	H	H	8.725
164	1	14	THR	N	N	121.866
165	1	15	LEU	H	H	8.640
166	1	15	LEU	N	N	125.088
167	1	16	GLU	H	H	8.108
168	1	16	GLU	N	N	122.786
169	1	17	VAL	H	H	8.917
170	1	17	VAL	N	N	117.749
171	1	18	GLU	H	H	8.630
172	1	18	GLU	N	N	119.401
173	1	20	SER	H	H	7.003
174	1	20	SER	N	N	103.525
175	1	21	ASP	H	H	8.033
176	1	21	ASP	N	N	124.103
177	1	22	THR	H	H	7.858
178	1	22	THR	N	N	109.129
179	1	23	ILE	H	H	8.495
180	1	23	ILE	N	N	121.543
181	1	25	ASN	H	H	7.904
182	1	25	ASN	N	N	121.427
183	1	26	VAL	H	H	8.106
184	1	26	VAL	N	N	122.552
185	1	27	LYS	H	H	8.494
186	1	27	LYS	N	N	119.073
187	1	28	ALA	H	H	7.958
188	1	28	ALA	N	N	123.429
189	1	29	LYS	H	H	7.905
190	1	29	LYS	N	N	120.622
191	1	30	ILE	H	H	8.233
192	1	30	ILE	N	N	121.781
193	1	31	GLN	H	H	8.453
194	1	31	GLN	N	N	123.419
195	1	32	ASP	H	H	8.130
196	1	32	ASP	N	N	120.245
197	1	33	LYS	H	H	7.526
198	1	33	LYS	N	N	115.791
199	1	34	GLU	H	H	8.601
200	1	34	GLU	N	N	113.553
201	1	35	GLY	H	H	8.480
202	1	35	GLY	N	N	109.192
203	1	39	ASP	H	H	8.517
204	1	39	ASP	N	N	113.827
205	1	40	GLN	H	H	7.755
206	1	40	GLN	N	N	116.970
207	1	41	GLN	H	H	7.458
208	1	41	GLN	N	N	118.013
209	1	42	ARG	H	H	8.453
210	1	42	ARG	N	N	123.419
211	1	43	LEU	H	H	8.738
212	1	43	LEU	N	N	124.728
213	1	44	ILE	H	H	9.138
214	1	44	ILE	N	N	122.673
215	1	45	PHE	H	H	8.791
216	1	45	PHE	N	N	124.936
217	1	46	ALA	H	H	8.963
218	1	46	ALA	N	N	133.183
219	1	47	GLY	H	H	8.115
220	1	47	GLY	N	N	102.679
221	1	48	LYS	H	H	7.925
222	1	48	LYS	N	N	122.115
223	1	49	GLN	H	H	8.635
224	1	49	GLN	N	N	123.259
225	1	50	LEU	H	H	8.534
226	1	50	LEU	N	N	125.722
227	1	51	GLU	H	H	8.389
228	1	51	GLU	N	N	123.340
229	1	52	ASP	H	H	8.163
230	1	52	ASP	N	N	120.653
231	1	54	ARG	H	H	7.441
232	1	54	ARG	N	N	119.577
233	1	55	THR	H	H	8.790
234	1	55	THR	N	N	108.870
235	1	56	LEU	H	H	8.128
236	1	56	LEU	N	N	118.171
237	1	57	SER	H	H	8.453
238	1	57	SER	N	N	113.583
239	1	58	ASP	H	H	7.916
240	1	58	ASP	N	N	124.699
241	1	59	TYR	H	H	7.228
242	1	59	TYR	N	N	115.935
243	1	60	ASN	H	H	8.119
244	1	60	ASN	N	N	115.961
245	1	61	ILE	H	H	7.234
246	1	61	ILE	N	N	119.171
247	1	62	GLN	H	H	7.640
248	1	62	GLN	N	N	125.047
249	1	63	LYS	H	H	8.450
250	1	63	LYS	N	N	120.600
251	1	64	GLU	H	H	9.286
252	1	64	GLU	N	N	115.431
253	1	65	SER	H	H	7.732
254	1	65	SER	N	N	115.204
255	1	66	THR	H	H	8.670
256	1	66	THR	N	N	117.513
257	1	67	LEU	H	H	9.384
258	1	67	LEU	N	N	128.045
259	1	68	HIS	H	H	9.198
260	1	68	HIS	N	N	119.763
261	1	69	LEU	H	H	8.314
262	1	69	LEU	N	N	124.082
263	1	70	VAL	H	H	9.145
264	1	70	VAL	N	N	126.987
265	1	71	LEU	H	H	8.203
266	1	71	LEU	N	N	124.144
267	1	72	ARG	H	H	8.550
268	1	72	ARG	N	N	124.225
269	1	73	LEU	H	H	8.334
270	1	73	LEU	N	N	124.375
271	1	74	ARG	H	H	8.487
272	1	74	ARG	N	N	121.748
273	1	75	GLY	H	H	8.576
274	1	75	GLY	N	N	110.516
275	1	76	GLY	H	H	8.178
276	1	76	GLY	N	N	109.292

Protein Blocks Logo

Calculated from 1 models in PDB: 2MBQ, Strand ID: A, B Detail

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Release date

2013-08-15

Citation

Unique structural, dynamical, and functional properties of k11-linked polyubiquitin chains
Castaneda, C.A., Kashyap, T.R., Nakasone, M.A., Krueger, S., Fushman, D.
Structure (2013), 21, 1168-1181, PubMed 23823328 , DOI 10.1016/j.str.2013.04.029 , Abstract

Related entities 1. K11-linked Diubiquitin, : 203 : 6 : 85 : 224 entities Detail

Interaction partners 1. K11-linked Diubiquitin, : 2 interactors Detail

Experiments performed 2 experiments Detail