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BMRB: 19415

2MBV

LMO4-LIM2 in complex with DEAF-1 (404-418)

Authors

Joseph, S., Matthews, J.M., Kwan, A.H-Y., Mackay, J.P., Cubeddu, L., Foo, P.

Assembly

LMO4-LIM2 in complex with DEAF-1 (404-418)

Entity

1. DIC4 (polymer), 96 monomers, 10130.36 Da Detail

GSYIRLFGNS GACSACGQSI PASELVMRAQ GNVYHLKCFT CSTCRNRLVP GDRFHYINGS LFCEHDRPTA LINGGSGGSG SIAPFPEAAL PTSHPK

2. LMO4-LIM2 in complex with DEAF-1 (404-418), entity ZN (non-polymer), 65.409 × 2 Da

Total weight

10261.179 Da

Max. entity weight

10130.36 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics, matrix relaxation, torsion angle dynamics, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 93.8 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.8 % (975 of 1040)	96.8 % (521 of 538)	88.9 % (361 of 406)	96.9 % (93 of 96)
Backbone	96.8 % (544 of 562)	94.4 % (186 of 197)	98.2 % (271 of 276)	97.8 % (87 of 89)
Sidechain	91.6 % (515 of 562)	98.2 % (335 of 341)	81.3 % (174 of 214)	85.7 % (6 of 7)
Aromatic	55.6 % (50 of 90)	100.0 % (45 of 45)	11.1 % (5 of 45)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. DIC4

GSYIRLFGNS GACSACGQSI PASELVMRAQ GNVYHLKCFT CSTCRNRLVP GDRFHYINGS LFCEHDRPTA LINGGSGGSG SIAPFPEAAL PTSHPK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 5.0 (±.2), Details pH 5.0

#	Name	Isotope labeling	Concentration
1	DIC4	[U-99% 13C; U-99% 15N]	0.3 ~ 0.7 (±0.1) mM
2	acetic acid	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	35 (±1.0) mM
4	DTT	natural abundance	1 (±0.1) mM
5	DSS	natural abundance	.067 (±0.01) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MBV, Strand ID: A Detail

Release date

2014-02-12

Citation

Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1
Joseph, S., Kwan, A.H.Y., Mackay, J.P., Cubeddu, L., Matthews, J.M.
Biomol. NMR Assign. (2014), 8, 141-144, PubMed 23417771 , DOI 10.1007/s12104-013-9470-x , Abstract

Related entities 1. DIC4, : 1 : 50 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC