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Found 1 Document (4.329 seconds)

BMRB: 19425

2MC2

X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A.

Authors

Amador, F., Stathopulos, P., Seabrook, G., Ikura, M.

Assembly

mouse RyR2 domain A

Entity

1. mouse RyR2 domain A (polymer, Thiol state: all free), 219 monomers, 24142.90 Da Detail

GSGSEIQFLR TDDEVVLQCT ATIHKEQQKL CLAAEGFGNR LCFLESTSNS KNVPPDLSIC TFVLEQSLSV RALQEMLANT VEKSEGQVDV EKWKFMMKTA QGGGHRTLLY GHAILLRHSY SGMYLCCLST SRSSTDKLAF DVGLQEDTTG EACWWTIHPA SKQRSEGEKV RVGDDLILVS VSSERYLHLS YGNSSWHVDA AFQQTLWSVA PISSGSEAA

Formula weight

24142.9 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.1 %, Completeness: 42.8 %, Completeness (bb): 73.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.8 % (1059 of 2472)	23.1 % (294 of 1271)	59.9 % (577 of 964)	79.3 % (188 of 237)
Backbone	73.4 % (959 of 1306)	48.9 % (220 of 450)	86.0 % (551 of 641)	87.4 % (188 of 215)
Sidechain	20.3 % (278 of 1369)	9.4 % (77 of 821)	38.2 % (201 of 526)	0.0 % (0 of 22)
Aromatic	5.1 % (10 of 198)	5.1 % (5 of 99)	5.3 % (5 of 94)	0.0 % (0 of 5)
Methyl	19.0 % (46 of 242)	16.5 % (20 of 121)	21.5 % (26 of 121)

1. entity

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7, Details wild-type RyR2A 10-224

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N; U-2H]	0.4 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	TCEP	natural abundance	2 mM
5	DTT	natural abundance	5 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.54 ppm, Outliers: 7 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MC2, Strand ID: A Detail

Release date

2013-09-09

Citation

Type 2 ryanodine receptor domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy
Amador, F.J., Kimlicka, L., Stathopulos, P.B., Gasmi-Seabrook, G.M.C., Maclennan, D.H., Van Petegem, F., Ikura, M.
J. Mol. Biol. (2013), 425, 4034-4046, PubMed 23978697 , DOI 10.1016/j.jmb.2013.08.015 , Abstract

Related entities 1. mouse RyR2 domain A, : 1 : 25 : 78 entities Detail

Interaction partners 1. mouse RyR2 domain A, : 20 interactors Detail

More details for 20 interactors identified by 8 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC