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BMRB: 19427

2MC4

The solution structure of the C-terminal domain of BldD from Streptomyces coelicolor

Authors

Kim, J., Won, H., Kang, S.

Assembly

BldD-CTD monomer

Entity

1. BldD-CTD monomer (polymer, Thiol state: not present), 89 monomers, 9842.034 Da Detail

MEPPPKLVLD LERLATVPAE KAGPLQRYAA TIQSQRGDYN GKVLSIRQDD LRTLAVIYDQ SPSVLTEQLI SWGVLDADAR RAVASHDEL

Formula weight

9842.034 Da

Source organism

Streptomyces coelicolor

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 94.6 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (959 of 1014)	93.7 % (494 of 527)	94.9 % (376 of 396)	97.8 % (89 of 91)
Backbone	96.0 % (501 of 522)	96.0 % (169 of 176)	95.4 % (251 of 263)	97.6 % (81 of 83)
Sidechain	93.8 % (541 of 577)	92.6 % (325 of 351)	95.4 % (208 of 218)	100.0 % (8 of 8)
Aromatic	90.0 % (36 of 40)	90.0 % (18 of 20)	89.5 % (17 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. BldD-CTD

MEPPPKLVLD LERLATVPAE KAGPLQRYAA TIQSQRGDYN GKVLSIRQDD LRTLAVIYDQ SPSVLTEQLI SWGVLDADAR RAVASHDEL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	BldD-CTD	[U-99% 13C; U-98% 15N]	2 mM
2	sodium phosphate	natural abundance	40 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.005 %
5	H2O	natural abundance	93 %
6	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MC4, Strand ID: A Detail

Release date

2013-11-25

Citation

The C-terminal domain of the transcriptional regulator BldD from Streptomyces coelicolor A3(2) constitutes a novel fold of winged-helix domains
Kim, J., Won, H., Kang, S.
Proteins (2014), 82, 1093-1098, PubMed 24356916 , DOI 10.1002/prot.24481 , Abstract