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BMRB: 19432

2MC9

Cat r 1

Authors

Mueller, G., London, R., Ghosh, D.

Assembly

Cat r 1

Entity

1. Cat r 1 (polymer, Thiol state: all free), 177 monomers, 18764.02 Da Detail

SFTGSMPNPR VFFDMSVGGQ PAGRIVMELF ADTTPRTAEN FRALCTGEKG TGRSGKPLHY KDSSFHRVIP GFMCQGGDFT AGNGTGGESI YGAKFADENF IKKHTGPGIL SMANAGPNTN GSQFFICTAK TEWLDGKHVV FGQVVEGMDV VKAIEKVGSS SGRTAKKVVV EDCGQLS

Formula weight

18764.02 Da

Source organism

Catharanthus roseus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 77.4 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.4 % (1514 of 1956)	79.8 % (807 of 1011)	70.8 % (540 of 763)	91.8 % (167 of 182)
Backbone	83.7 % (875 of 1046)	96.8 % (360 of 372)	69.1 % (349 of 505)	98.2 % (166 of 169)
Sidechain	74.3 % (788 of 1061)	70.0 % (447 of 639)	83.1 % (340 of 409)	7.7 % (1 of 13)
Aromatic	34.5 % (60 of 174)	37.9 % (33 of 87)	30.2 % (26 of 86)	100.0 % (1 of 1)
Methyl	98.7 % (156 of 158)	98.7 % (78 of 79)	98.7 % (78 of 79)

1. entity

SFTGSMPNPR VFFDMSVGGQ PAGRIVMELF ADTTPRTAEN FRALCTGEKG TGRSGKPLHY KDSSFHRVIP GFMCQGGDFT AGNGTGGESI YGAKFADENF IKKHTGPGIL SMANAGPNTN GSQFFICTAK TEWLDGKHVV FGQVVEGMDV VKAIEKVGSS SGRTAKKVVV EDCGQLS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MC9, Strand ID: A Detail

Release date

2014-06-22

Citation

Primary identification, biochemical characterization, and immunologic properties of the allergenic pollen cyclophilin Cat r 1
Ghosh, D., Mueller, G., Schramm, G., Edwards, L., London, R., Petersen, A., Haas, H., Bhattacharya, S.
Not known

Related entities 1. Cat r 1, : 1 : 1 : 306 entities Detail

Interaction partners 1. Cat r 1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC