1H, 13C, and 15N Chemical Shift Assignments for Sp140 PHD finger cis conformer
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS11:SG | 2:ZN1:ZN |
2 | metal coordination | sing | 1:CYS14:SG | 2:ZN1:ZN |
3 | metal coordination | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | metal coordination | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
5 | disulfide | sing | 1:CYS22:SG | 1:CYS34:SG |
6 | metal coordination | sing | 1:CYS23:SG | 2:ZN1:ZN |
7 | metal coordination | sing | 1:CYS26:SG | 2:ZN1:ZN |
8 | metal coordination | sing | 1:CYS48:SG | 2:ZN1:ZN |
9 | metal coordination | sing | 1:CYS51:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.9 % (515 of 621) | 91.7 % (298 of 325) | 68.8 % (165 of 240) | 92.9 % (52 of 56) |
Backbone | 94.5 % (312 of 330) | 91.2 % (103 of 113) | 95.7 % (157 of 164) | 98.1 % (52 of 53) |
Sidechain | 74.3 % (255 of 343) | 92.0 % (195 of 212) | 46.9 % (60 of 128) | 0.0 % (0 of 3) |
Aromatic | 60.0 % (30 of 50) | 100.0 % (25 of 25) | 20.8 % (5 of 24) | 0.0 % (0 of 1) |
Methyl | 47.2 % (17 of 36) | 83.3 % (15 of 18) | 11.1 % (2 of 18) |
1. Sp140 PHD finger cis conformer
GAMGMRNLDE CEVCRDGGEL FCCDTCSRVF HEDCHIPPVE AERTPWNCIF CRMKESSolvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sp140 PHD finger | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | sodium phosphate buffer pH 6.3 | natural abundance | 20 mM | |
3 | NaCl | natural abundance | 150 mM | |
4 | DTT | natural abundance | 4 mM | |
5 | ZnCl2 | natural abundance | 50 uM | |
6 | DSS | natural abundance | 0.3 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Sp140 PHD finger | [U-100% 13C; U-100% 15N] | 1 mM | |
10 | sodium phosphate buffer pH 6.3 | natural abundance | 20 mM | |
11 | NaCl | natural abundance | 150 mM | |
12 | DTT | natural abundance | 4 mM | |
13 | ZnCl2 | natural abundance | 50 uM | |
14 | DSS | natural abundance | 0.3 mM | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | Sp140 PHD finger | [U-100% 15N] | 1 mM | |
18 | sodium phosphate buffer pH 6.3 | natural abundance | 20 mM | |
19 | NaCl | natural abundance | 150 mM | |
20 | DTT | natural abundance | 4 mM | |
21 | ZnCl2 | natural abundance | 50 uM | |
22 | DSS | natural abundance | 0.3 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | Sp140 PHD finger | natural abundance | 1 mM | |
26 | sodium phosphate buffer pH 6.3 | natural abundance | 20 mM | |
27 | NaCl | natural abundance | 150 mM | |
28 | DTT | natural abundance | 4 mM | |
29 | ZnCl2 | natural abundance | 50 uM | |
30 | DSS | natural abundance | 0.3 mM | |
31 | H2O | natural abundance | 90 % | |
32 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | Sp140 PHD finger | natural abundance | 1 mM | |
34 | sodium phosphate buffer pH 6.3 | natural abundance | 20 mM | |
35 | NaCl | natural abundance | 150 mM | |
36 | DTT | natural abundance | 4 mM | |
37 | ZnCl2 | natural abundance | 50 uM | |
38 | DSS | natural abundance | 0.3 mM | |
39 | H2O | natural abundance | 90 % | |
40 | D2O | natural abundance | 10 % |