BMRB: 19505

WW Domain Strand-Swapped Dimer

Authors

Kier, B.L., Sheffler, W., Baker, D.

Assembly

WW Domain Strand-Swapped Dimer

Entity

1. WW Domain Strand-Swapped Dimer (polymer), 27 monomers, 3356.812 × 2 Da Detail

KLPPGWEKRC FYFNRITGKR QFERPSD

Total weight

6713.624 Da

Max. entity weight

3356.812 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.3 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.3 % (179 of 194)	92.3 % (179 of 194)
Backbone	98.1 % (52 of 53)	98.1 % (52 of 53)
Sidechain	90.1 % (127 of 141)	90.1 % (127 of 141)
Aromatic	100.0 % (25 of 25)	100.0 % (25 of 25)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. entity

KLPPGWEKRC FYFNRITGKR QFERPSD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 6.5, Details 1 mM protein (0.5 mM dimer)

#	Name	Isotope labeling	Type	Concentration
1	WW Domain Strand-Swapped Dimer	natural abundance		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance		50 mM
3	DSS	natural abundance		0.5 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 33 models in PDB: 2MDW, Strand ID: A, B Detail

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Release date

2014-09-28

Citation

Covalent Assembly of Homooligomeric Proteins Using Structure-templating Hubs
Kier, B.L., Sheffler, W., Baker, D.
Biochemistry

Related entities 1. WW Domain Strand-Swapped Dimer, : 1 : 180 entities Detail

Experiments performed 2 experiments Detail