BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	97.6 % (369 of 378)	99.0 % (193 of 195)	96.7 % (145 of 150)	93.9 % (31 of 33)
Backbone	96.9 % (157 of 162)	96.3 % (52 of 54)	98.8 % (80 of 81)	92.6 % (25 of 27)
Sidechain	98.4 % (239 of 243)	100.0 % (141 of 141)	95.8 % (92 of 96)	100.0 % (6 of 6)
Aromatic	94.9 % (74 of 78)	100.0 % (39 of 39)	88.2 % (30 of 34)	100.0 % (5 of 5)
Methyl	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)

1. MPER-PB7

EKDLLALDKW NSLWSWFDIT KWLWYIK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N/13C-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
1	MPER-PB7	[U-100% 13C; U-100% 15N]	1 mM
2	DPC	[U-100% 2H]	100 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details unlabeled MPER-PB7

#	Name	Isotope labeling	Concentration
9	MPER-PB7	natural abundance	1 mM
10	DPC	[U-100% 2H]	100 mM
11	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2ME4, Strand ID: A Detail

Release date

2013-10-07

Citation

Disruption of helix-capping residues 671 and 674 reveals a role in HIV-1 entry for a specialized hinge segment of the membrane proximal external region of gp41
Sun, Z.J., Cheng, Y., Kim, M., Song, L., Choi, J., Kudahl, U.J., Brusic, V., Chowdhury, B., Yu, L., Seaman, M.S., Bellot, G., Shih, W.M., Wagner, G., Reinherz, E.L.
J. Mol. Biol. (2014), 426, 1095-1108, PubMed 24075869 , DOI 10.1016/j.jmb.2013.09.030 , Abstract

Related entities 1. MPER-PB7, : 1 : 106 entities Detail

Experiments performed 7 experiments Detail

1 3D backbone

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N/13C-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
1	MPER-PB7	[U-100% 13C; U-100% 15N]	1 mM
2	DPC	[U-100% 2H]	100 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

2 3D C(CO)NH,H(CCO)NH,HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N/13C-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
1	MPER-PB7	[U-100% 13C; U-100% 15N]	1 mM
2	DPC	[U-100% 2H]	100 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

3 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N/13C-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
1	MPER-PB7	[U-100% 13C; U-100% 15N]	1 mM
2	DPC	[U-100% 2H]	100 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
5	MPER-PB7	[U-100% 15N]	1 mM
6	DPC	[U-100% 2H]	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N-labeled MPER-PB7

#	Name	Isotope labeling	Concentration
5	MPER-PB7	[U-100% 15N]	1 mM
6	DPC	[U-100% 2H]	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 2D 1H-1H NOESY,TOCSY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details unlabeled MPER-PB7

#	Name	Isotope labeling	Concentration
9	MPER-PB7	natural abundance	1 mM
10	DPC	[U-100% 2H]	100 mM
11	D2O	natural abundance	100 %

7 Q-J RDC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.6 (±0.1), Details 15N/13C-labeled MPER-PB7 for RDC

#	Name	Isotope labeling	Concentration
12	MPER-PB7	[U-100% 13C; U-100% 15N]	1 mM
13	DPC	[U-100% 2H]	100 mM
14	DNA nanotube	natural abundance	20 mg/mL
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19515_2me4.nef
Input source #2: Coordindates	2me4.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---660-----670-------680---
EKDLLALDKWNSLWSWFDITKWLWYIK
|||||||||||||||||||||||||||
EKDLLALDKWNSLWSWFDITKWLWYIK
--------10--------20-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	27	0	0	100.0

Content subtype: combined_19515_2me4.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	369		100.0 (chain: A, length: 27)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	555 (1)	noe	96.3 (chain: A, length: 27)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	46 (46)	.	96.3 (chain: A, length: 27)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 27, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 193
  - ¹³C chemical shifts: 145
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	195	193	99.0
¹³C chemical shifts	150	145	96.7
¹⁵N chemical shifts	33	31	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	52	96.3
¹³C chemical shifts	54	53	98.1
¹⁵N chemical shifts	27	25	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	141	141	100.0
¹³C chemical shifts	96	92	95.8
¹⁵N chemical shifts	6	6	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	16	100.0
¹³C chemical shifts	16	16	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	39	100.0
¹³C chemical shifts	34	30	88.2
¹⁵N chemical shifts	5	5	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 27, Sequence coverage: 96.3%
- Total number of restraints: 554
- Weight of restraints: 1.0 (554)
- Potential type of restraints: square-well-parabolic (554)
- Range of distance target values: 1.32, 4.33 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 27, Sequence coverage: 96.3%
- Total number of restraints: 46
- Total number of restraints per polymer type: 46
- Weight of restraints: 1.0 (46)
- Potential type of restraints: square-well-parabolic (46)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords helix-hinge-helix, MPER, viral fusion

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Found 1 Document (0.554 seconds)

BMRB: 19515

Sample #1

Sample #2

Related entities 1. MPER-PB7, : 1 : 106 entities Detail

Experiments performed 7 experiments Detail

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NMR combined restraints 4 contents Detail