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BMRB: 19540

2MF8

haddock model of MyT1 F4F5 - DNA complex

Authors

Gamsjaeger, R., O'Connell, M.R., Cubeddu, L., Shepherd, N.E., Lowry, J.A., Kwan, A.H., Vandevenne, M., Swanton, M.K., Matthews, J.M., Mackay, J.P.

Assembly

MyT1 F4F5 - DNA complex

Entity

1. MyT1 F4F5 - DNA complex, entity 1 (polymer), 93 monomers, 9563.754 Da Detail

GPLGSFDIKK ELLTCPTPGC DGSGHITGNY ASHRSLSGCP LADKSLRNLM AAHSADLKCP TPGCDGSGHI TGNYASHRSL SGCPRAKKSG LRV

2. DNA 26 mer (polymer, Thiol state: not present), 26 monomers, 8050.136 Da Detail

ACCGAAAGTT CACGTGAACT TTCGGT

3. MyT1 F4F5 - DNA complex, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

17744.709 Da

Max. entity weight

9563.754 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 55.5 %, Completeness: 20.2 %, Completeness (bb): 31.8 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	20.2 % (249 of 1230)	9.3 % (71 of 761)	32.9 % (125 of 380)	59.6 % (53 of 89)
Backbone	31.8 % (231 of 726)	15.5 % (58 of 374)	45.1 % (120 of 266)	61.6 % (53 of 86)
Sidechain	12.2 % (71 of 584)	3.4 % (13 of 387)	29.9 % (58 of 194)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 98)	0.0 % (0 of 75)	0.0 % (0 of 23)
Methyl	12.6 % (11 of 87)	6.4 % (3 of 47)	20.0 % (8 of 40)

1. entity 1

GPLGSFDIKK ELLTCPTPGC DGSGHITGNY ASHRSLSGCP LADKSLRNLM AAHSADLKCP TPGCDGSGHI TGNYASHRSL SGCPRAKKSG LRV

2. DNA 26 mer

ACCGAAAGTT CACGTGAACT TTCGGT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Myelin transcription factor 1	[U-13C; U-15N]	0.2 ~ 1.0 mM
2	DNA (26-MER)	natural abundance	0.2 ~ 1.0 mM
3	phosphate	natural abundance	10 mM
4	NaCl	natural abundance	50 mM
5	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2MF8, Strand ID: A Detail

Release date

2013-11-03

Citation

A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers
Gamsjaeger, R., Cubeddu, M.R., Shepherd, L., Lowry, N.E., Kwan, J.A., Vandevenne, A.H., Swanton, M., Matthews, M.K., Mackay, J.M.
J. Biol. Chem. (2013), 288, 35180-35191, PubMed 24097990 , DOI 10.1074/jbc.M113.482075 , Abstract

Related entities 1. MyT1 F4F5 - DNA complex, entity 1, : 1 : 66 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC