BMRB: 19541

Structure of the Nucleoplasmin-like N-terminal domain of Drosophila FKBP39

Authors

Artero, J., Forsyth, T., Callow, P., Watson, A.A., Zhang, W., Laue, E.D., EDLICH-MUTH, C.H., Przewloka, M., Edlich- muth, C.

Assembly

Structure of the Nucleoplasmin-like N-terminal domain of Drosophila FKBP39

Entity

1. Structure of the Nucleoplasmin-like N-terminal domain of Drosophila FKBP39 (polymer, Thiol state: not present), 98 monomers, 10808.31 × 5 Da Detail

FQGAMAMFWG LNMKPERKYS QTIIKSFHIS GVALDKGQEA KLYLAAEKQE YIVATVTKAI PQVALDLNFS KGDRIMFYTA GDASVSLLGY LHDIDSGS

Total weight

54041.547 Da

Max. entity weight

10808.31 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.0 %, Completeness: 40.8 %, Completeness (bb): 68.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	40.8 % (467 of 1144)	29.2 % (172 of 589)	45.0 % (203 of 451)	88.5 % (92 of 104)
Backbone	68.0 % (397 of 584)	55.4 % (112 of 202)	67.8 % (194 of 286)	94.8 % (91 of 96)
Sidechain	23.7 % (154 of 650)	15.5 % (60 of 387)	36.5 % (93 of 255)	12.5 % (1 of 8)
Aromatic	5.5 % (6 of 110)	9.1 % (5 of 55)	0.0 % (0 of 54)	100.0 % (1 of 1)
Methyl	31.6 % (36 of 114)	36.8 % (21 of 57)	26.3 % (15 of 57)

1. 39 KDA FK506-BINDING NUCLEAR PROTEIN

FQGAMAMFWG LNMKPERKYS QTIIKSFHIS GVALDKGQEA KLYLAAEKQE YIVATVTKAI PQVALDLNFS KGDRIMFYTA GDASVSLLGY LHDIDSGS

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Sample

Solvent system 90% water/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details 0.5mM

#	Name	Isotope labeling	Type	Concentration
1	39 KDA FK506-BINDING NUCLEAR PROTEIN	[U-13C; U-15N]		0.5 mM
2	MgCl2	natural abundance		25 mM

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Chem. Shift Complete2

Sequence coverage: 95.9 %, Completeness: 49.6 %, Completeness (bb): 66.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	49.6 % (1134 of 2288)	49.6 % (584 of 1178)	39.8 % (359 of 902)	91.8 % (191 of 208)
Backbone	66.5 % (777 of 1168)	73.3 % (296 of 404)	52.1 % (298 of 572)	95.3 % (183 of 192)
Sidechain	36.4 % (473 of 1300)	37.2 % (288 of 774)	34.9 % (178 of 510)	43.8 % (7 of 16)
Aromatic	25.0 % (55 of 220)	47.3 % (52 of 110)	0.9 % (1 of 108)	100.0 % (2 of 2)
Methyl	53.9 % (123 of 228)	59.6 % (68 of 114)	48.2 % (55 of 114)

1. 39 KDA FK506-BINDING NUCLEAR PROTEIN

FQGAMAMFWG LNMKPERKYS QTIIKSFHIS GVALDKGQEA KLYLAAEKQE YIVATVTKAI PQVALDLNFS KGDRIMFYTA GDASVSLLGY LHDIDSGS

Show sample condition

Sample

Solvent system 90% water/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details 0.5mM

#	Name	Isotope labeling	Type	Concentration
1	39 KDA FK506-BINDING NUCLEAR PROTEIN	[U-13C; U-15N]		0.5 mM
2	MgCl2	natural abundance		25 mM

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Chem. Shift Complete3

Sequence coverage: 100.0 %, Completeness: 61.0 %, Completeness (bb): 71.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	61.0 % (2094 of 3432)	63.3 % (1118 of 1767)	50.5 % (683 of 1353)	93.9 % (293 of 312)
Backbone	71.2 % (1248 of 1752)	81.0 % (491 of 606)	55.9 % (480 of 858)	96.2 % (277 of 288)
Sidechain	53.0 % (1034 of 1950)	54.0 % (627 of 1161)	51.2 % (392 of 765)	62.5 % (15 of 24)
Aromatic	47.3 % (156 of 330)	63.6 % (105 of 165)	29.6 % (48 of 162)	100.0 % (3 of 3)
Methyl	69.0 % (236 of 342)	72.5 % (124 of 171)	65.5 % (112 of 171)

1. 39 KDA FK506-BINDING NUCLEAR PROTEIN

FQGAMAMFWG LNMKPERKYS QTIIKSFHIS GVALDKGQEA KLYLAAEKQE YIVATVTKAI PQVALDLNFS KGDRIMFYTA GDASVSLLGY LHDIDSGS

Show sample condition

Sample

Solvent system 90% water/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details 0.5mM

#	Name	Isotope labeling	Type	Concentration
1	39 KDA FK506-BINDING NUCLEAR PROTEIN	[U-13C; U-15N]		0.5 mM
2	MgCl2	natural abundance		25 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 4CA9, Strand ID: A, B, C, D, E Detail

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Release date

2014-10-26

Related entities 1. Structure of the Nucleoplasmin-like N-terminal domain of Drosophila FKBP39, : 1 : 19 entities Detail

Interaction partners 1. Structure of the Nucleoplasmin-like N-terminal domain of Drosophila FKBP39, : 8 interactors Detail

Experiments performed 14 experiments Detail