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BMRB: 19554

2MFL

Domain 2 of E. coli ribosomal protein S1

Authors

GIRAUD, P., CRECHET, J., BONTEMS, F., UZAN, M., SIZUN, C.

Assembly

Domain 2 of E. coli ribosomal protein S1

Entity

1. Domain 2 of E. coli ribosomal protein S1 (polymer, Thiol state: not present), 182 monomers, 19957.27 Da Detail

GSHMTESFAQ LFEESLKEIE TRPGSIVRGV VVAIDKDVVL VDAGLKSESA IPAEQFKNAQ GELEIQVGDE VDVALDAVED GFGETLLSRE KAKRHEAWIT LEKAYEDAET VTGVINGKVK GGFTVELNGI RAFLPGSLVD VRPVRDTLHL EGKELEFKVI KLDQKRNNVV VSRRAVIESE NS

Formula weight

19957.27 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 86.5 %, Completeness (bb): 93.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.5 % (1792 of 2072)	84.7 % (908 of 1072)	87.3 % (707 of 810)	93.2 % (177 of 190)
Backbone	93.4 % (1012 of 1084)	94.7 % (355 of 375)	92.5 % (491 of 531)	93.3 % (166 of 178)
Sidechain	80.8 % (933 of 1155)	79.3 % (553 of 697)	82.7 % (369 of 446)	91.7 % (11 of 12)
Aromatic	69.6 % (71 of 102)	94.1 % (48 of 51)	44.0 % (22 of 50)	100.0 % (1 of 1)
Methyl	92.1 % (221 of 240)	92.5 % (111 of 120)	91.7 % (110 of 120)

1. S1F12

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1.000 (±0.01) atm, Temperature 293.000 (±0.50) K, pH 6.800 (±0.10)

#	Name	Isotope labeling	Concentration
1	S1F12	[U-99% 13C; U-99% 15N; U-80% 2H]	0.25 (±0.025) mM
2	sodium phosphate	natural abundance	25.0 (±1.0) mM
3	sodium chloride	natural abundance	200.0 (±2.0) mM
4	H2O	natural abundance	93 %
5	D2O	[U-100% 2H]	7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1.000 (±0.01) atm, Temperature 293.000 (±0.50) K, pH 6.800 (±0.10)

#	Name	Isotope labeling	Concentration
6	S1F12	[U-99% 13C; U-99% 15N]	0.2 (±0.02) mM
7	potassium phosphate	natural abundance	50.0 (±1.0) mM
8	potassium chloride	natural abundance	200.0 (±2.0) mM
9	H2O	natural abundance	93 %
10	D2O	[U-100% 2H]	7 %

Sample #3

Solvent system 100% D2O, Pressure 1.000 (±0.01) atm, Temperature 293.000 (±0.50) K, pH 6.800 (±0.10)

#	Name	Isotope labeling	Concentration
11	S1F12	[U-99% 13C; U-99% 15N]	0.2 (±0.02) mM
12	potassium phosphate	natural abundance	50.0 (±1.0) mM
13	potassium chloride	natural abundance	200.0 (±2.0) mM
14	D2O	[U-100% 2H]	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MFL, Strand ID: A Detail

Release date

2014-04-13

Citation

Resonance assignment of the ribosome binding domain of E. coli ribosomal protein S1
Giraud, P., Crechet, J., Uzan, M., Bontems, F., Sizun, C.
Biomol. NMR Assign. (2015), 9, 107-111, PubMed 24682851 , DOI 10.1007/s12104-014-9554-2 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19550 released on 2014-04-13
Title Domain 1 of E. coli ribosomal protein S1

Related entities 1. Domain 2 of E. coli ribosomal protein S1, : 1 : 5 : 54 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC