BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	92.6 % (629 of 679)	92.8 % (323 of 348)	93.9 % (245 of 261)	87.1 % (61 of 70)
Backbone	97.9 % (376 of 384)	97.8 % (132 of 135)	97.9 % (183 of 187)	98.4 % (61 of 62)
Sidechain	88.1 % (310 of 352)	89.7 % (191 of 213)	90.8 % (119 of 131)	0.0 % (0 of 8)
Aromatic	50.0 % (9 of 18)	100.0 % (9 of 9)	0.0 % (0 of 9)
Methyl	96.2 % (77 of 80)	97.5 % (39 of 40)	95.0 % (38 of 40)

1. PonA2-PASTA

GSRVPSVAGL DVDAARQRLK DAGFQVADQT NSVNSSAKYG EVVGTSPSGQ TIPGSIVTIQ ISNGI

Show sample condition

Sample #1

Solvent system 90% phosphate buffer /10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details sodium phosphate 30 mM, pH 6.4, sodium azide 0.02%

#	Name	Isotope labeling	Concentration
1	PonA2-PASTA	natural abundance	1.5 ~ 1.6 mM
2	D2O	[U-100% 2H]	10 %
3	sodium phosphate	natural abundance	30 mM
4	sodium azide	natural abundance	0.02 %
5	Phosphate buffer	natural abudnance	90 %

Sample #2

Solvent system 90% phosphate buffer /10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details sodium phosphate 30 mM, pH 6.4, sodium azide 0.02%

#	Name	Isotope labeling	Concentration
6	PonA2-PASTA	[U-100% 15N]	1.8 ~ 1.9 mM
7	D2O	[U-100% 2H]	10 %
8	sodium phosphate	natural abundance	30 mM
9	sodium azide	natural abundance	0.02 %
10	Phosphate buffer	natural abudnance	90 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details sodium phosphate 30 mM, pH 6.4, sodium azide 0.02%

#	Name	Isotope labeling	Concentration
11	PonA2-PASTA	[U-100% 13C; U-100% 15N]	1.3 mM
12	D2O	natural abundance	10 %
13	sodium phosphate	natural abundance	30 mM
14	sodium azide	natural abundance	0.02 %
15	H2O	natural abudnance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.25
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1

Referencing offset: 0.19 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.25
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1

Referencing offset: 0.19 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.25
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.25
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MGV, Strand ID: A Detail

Release date

2013-12-22

Citation

Structural and binding properties of the PASTA domain of PonA2, a key penicillin binding protein from Mycobacterium tuberculosis
Calvanese, L., Falcigno, L., Maglione, C., Marasco, D., Ruggiero, A., Squeglia, F., Berisio, R.
Biopolymers (2014), 101, 712-719, PubMed 24281824 , DOI 10.1002/bip.22447 , Abstract

Related entities 1. PASTA domain of PonA2, : 1 : 1 : 24 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC