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BMRB: 19613

2MH2

Structural insights into the DNA recognition and protein interaction domains reveal fundamental homologous DNA pairing properties of HOP2

Authors

Moktan, H., Guiraldelli, M.F., Eyter, C.A., Zhao, W., Camerini-Otero, R.D., Sung, P., Zhou, D.H., Pezza, R.J.

Assembly

HOP2

Entity

1. HOP2 (polymer, Thiol state: not present), 84 monomers, 9244.286 Da Detail

MSKSRAEAAA GAPGIILRYL QEQNRPYSAQ DVFGNLQKEH GLGKAAVVKA LDQLAQEGKI KEKTYGKQKI YFADQNQFDT VSDA

Formula weight

9244.286 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 83.2 %, Completeness (bb): 93.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.2 % (815 of 979)	81.3 % (418 of 514)	84.6 % (314 of 371)	88.3 % (83 of 94)
Backbone	93.4 % (467 of 500)	93.1 % (161 of 173)	93.9 % (230 of 245)	92.7 % (76 of 82)
Sidechain	75.7 % (421 of 556)	75.4 % (257 of 341)	77.3 % (157 of 203)	58.3 % (7 of 12)
Aromatic	0.0 % (0 of 66)	0.0 % (0 of 33)	0.0 % (0 of 33)
Methyl	97.6 % (82 of 84)	95.2 % (40 of 42)	100.0 % (42 of 42)

1. Hop2

MSKSRAEAAA GAPGIILRYL QEQNRPYSAQ DVFGNLQKEH GLGKAAVVKA LDQLAQEGKI KEKTYGKQKI YFADQNQFDT VSDA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.8

#	Name	Isotope labeling	Concentration
1	Hop2	[U-99% 13C; U-99% 15N]	6 mg/mL
2	D2O	[U-99% 2H]	5 %
3	imidazole	natural abundance	10 mM
4	sodium chloride	natural abundance	120 mM
5	glycerol	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MH2, Strand ID: A Detail

Release date

2014-04-10

Citation

Solution structure and DNA-binding properties of the winged helix domain of the meiotic recombination HOP2 protein
Moktan, H., Guiraldelli, M.F., Eyster, C.A., Zhao, W., Lee, C., Mather, T., Camerini-Otero, R., Sung, P., Zhou, D.H., Pezza, R.J.
J. Biol. Chem. (2014), 289, 14682-14691, PubMed 24711446 , DOI 10.1074/jbc.M114.548180 , Abstract

Related entities 1. HOP2, : 1 : 2 : 2 : 32 entities Detail

Interaction partners 1. HOP2, : 15 interactors Detail

More details for 15 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC