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BMRB: 19627

2MHD

NMR strucutre of the hypothetical protein BACUNI_03114 from Bacteroides uniformis ATCC 8492

Authors

Shnitkind, S., Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

hypothetical protein BACUNI 03114 from Bacteroides uniformis ATCC 8492

Entity

1. hypothetical protein BACUNI 03114 from Bacteroides uniformis ATCC 8492 (polymer, Thiol state: all free), 110 monomers, 12797.16 Da Detail

GDEDDKVEIP QLVGKWIVKE PVLQDDFVTC YTFNADKTYE VYTGSPLSNG VPFRGTYIIS LDEKLIKLYD KEEHCTEQYH ILKLTSKEMK WENASPKDGN SDKRLEKYND

Formula weight

12797.16 Da

Source organism

Bacteroides uniformis

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.9 %, Completeness (bb): 86.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.9 % (1190 of 1324)	96.5 % (669 of 693)	78.7 % (406 of 516)	100.0 % (115 of 115)
Backbone	86.2 % (560 of 650)	98.2 % (217 of 221)	73.5 % (238 of 324)	100.0 % (105 of 105)
Sidechain	94.2 % (733 of 778)	95.8 % (452 of 472)	91.6 % (271 of 296)	100.0 % (10 of 10)
Aromatic	77.1 % (91 of 118)	88.1 % (52 of 59)	64.9 % (37 of 57)	100.0 % (2 of 2)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. entity

GDEDDKVEIP QLVGKWIVKE PVLQDDFVTC YTFNADKTYE VYTGSPLSNG VPFRGTYIIS LDEKLIKLYD KEEHCTEQYH ILKLTSKEMK WENASPKDGN SDKRLEKYND

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	sodium azide	natural abundance	5 mM
4	entity	[U-99% 13C; U-99% 15N]	1.2 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2MHD, Strand ID: A Detail

Release date

2013-12-19

Citation

NMR strucutre of the hypothetical protein BACUNI_03114 from Bacteroides uniformis ATCC 8492
Shnitkind, S., Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. hypothetical protein BACUNI 03114 from Bacteroides uniformis ATCC 8492, : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC