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BMRB: 19634

2MHI

Solution structure of the CR4/5 domain of medaka telomerase RNA

Authors

Kim, N., Zhang, Q., Feigon, J.

Assembly

CR4/5 domain of medaka telomerase RNA

Entity

1. CR4/5 domain of medaka telomerase RNA (polymer, Thiol state: not present), 53 monomers, 17055.00 Da Detail

GGAAACGCCG CGGUCAGCUC GGCUGCUGCG AAGAGUUCGU CUCUGUUGUU UCC

Formula weight

17055.0 Da

Source organism

Oryzias latipes

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.2 %, Completeness (bb): 93.7 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	93.2 % (795 of 853)	94.2 % (441 of 468)	94.6 % (335 of 354)	61.3 % (19 of 31)
Suger, PO4	93.7 % (546 of 583)	94.3 % (300 of 318)	92.8 % (246 of 265)
Nucleobase	92.2 % (249 of 270)	94.0 % (141 of 150)	100.0 % (89 of 89)	61.3 % (19 of 31)
Aromatic	91.7 % (220 of 240)	93.3 % (112 of 120)	100.0 % (89 of 89)	61.3 % (19 of 31)

1. CR4/5 domain of medaka telomerase RNA

GGAAACGCCG CGGUCAGCUC GGCUGCUGCG AAGAGUUCGU CUCUGUUGUU UCC

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	mdCR4/5	natural abundance	1.2 mM
2	sodium phosphate	natural abundance	10 mM
3	potassium chloride	natural abundance	100 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
4	mdCR4/5	natural abundance	1.2 mM
5	sodium phosphate	natural abundance	10 mM
6	potassium chloride	natural abundance	100 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
7	mdCR4/5	[U-13C; U-15N]	1 mM
8	sodium phosphate	natural abundance	10 mM
9	potassium chloride	natural abundance	100 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
10	mdCR4/5	[U-13C; U-15N]-Ade	1 mM
11	sodium phosphate	natural abundance	10 mM
12	potassium chloride	natural abundance	100 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
13	mdCR4/5	[U-13C; U-15N]-Ura	1 mM
14	sodium phosphate	natural abundance	10 mM
15	potassium chloride	natural abundance	100 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	mdCR4/5	[U-13C; U-15N]-Gua	1 mM
17	sodium phosphate	natural abundance	10 mM
18	potassium chloride	natural abundance	100 mM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
19	mdCR4/5	[U-13C; U-15N]-Cyt	1 mM
20	sodium phosphate	natural abundance	10 mM
21	potassium chloride	natural abundance	100 mM

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
22	mdCR4/5	[U-2H]	1.5 mM
23	sodium phosphate	natural abundance	10 mM
24	potassium chloride	natural abundance	100 mM

Sample #9

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.3

#	Name	Isotope labeling	Concentration
25	mdCR4/5	[U-2H]	1.5 mM
26	sodium phosphate	natural abundance	10 mM
27	potassium chloride	natural abundance	100 mM

Release date

2013-12-22

Citation

Structure and sequence elements of the CR4/5 domain of medaka telomerase RNA important for telomerase function
Kim, N., Zhang, Q., Feigon, J.
Nucleic Acids Res. (2014), 42, 3395-3408, PubMed 24335084 , DOI 10.1093/nar/gkt1276 , Abstract