BMRB: 19638

Solution structure of cytochrome c Y67H

Authors

Lan, W.X., Wang, Z.H., Ying, T.L., Yang, Z.Z., Zhang, X., Wu, H.M., Liu, M.L., Tian, X.S.

Assembly

cytochrome c Y67H

Entity

1. cytochrome c Y67H, entity 1 (polymer), 108 monomers, 11965.47 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EHLTNPAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

2. cytochrome c Y67H, entity HEC (non-polymer), 618.503 Da

Total weight

12583.973 Da

Max. entity weight

11965.47 Da

Entity Connection

metal coordination 2, covalent 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:HIS23:NE2	2:HEC1:FE
2	metal coordination	sing	1:MET85:SD	2:HEC1:FE
3	covalent	sing	1:CYS19:SG	2:HEC1:CAB
4	covalent	sing	1:CYS22:SG	2:HEC1:CAC

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 85.0 %, Completeness (bb): 93.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.0 % (563 of 662)	85.0 % (563 of 662)
Backbone	93.3 % (209 of 224)	93.3 % (209 of 224)
Sidechain	80.8 % (354 of 438)	80.8 % (354 of 438)
Aromatic	88.5 % (46 of 52)	88.5 % (46 of 52)
Methyl	93.6 % (44 of 47)	93.6 % (44 of 47)

1. entity 1

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EHLTNPAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0, Details 50mM phosphate buffer NA; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	cytc c y67h	natural abundance		1 mM
2	phosphate buffer NA	natural abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	cytc c y67h	natural abundance		1 mM
4	phosphate buffer NA	natural abundance		50 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2MHM, Strand ID: A Detail

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Release date

2014-10-26

Citation

Structural basis for cytochrome c Y67H mutant to function as a peroxidase
Lan, W., Wang, Z., Yang, Z., Ying, T., Zhang, X., Tan, X., Liu, M., Cao, C., Huang, Z.
PLoS One (2014), 9, e107305-e107305, PubMed 25210769 , DOI 10.1371/journal.pone.0107305 , Abstract

Related entities 1. cytochrome c Y67H, entity 1, : 1 : 3 : 4 : 228 entities Detail

Interaction partners 1. cytochrome c Y67H, entity 1, : 4 interactors Detail

Experiments performed 3 experiments Detail

NMR combined restraints 3 contents Detail