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BMRB: 19642

2MHN

NMR structure of the first RRM domain of the protein RBM39 from homo sapiens

Authors

Serrano, P., Geralt, M., Dutta, S.K., Wuthrich, K.

Assembly

RBM39 RRM1

Entity

1. RBM39 RRM1 (polymer, Thiol state: all free), 152 monomers, 16798.22 Da Detail

GHMNLTPEER DARTVFCMQL AARIRPRDLE EFFSTVGKVR DVRMISDRNS RRSKGIAYVE FVDVSSVPLA IGLTGQRVLG VPIIVQASQA EKNRRRSKGI AYVEFVDVSS VPLAIGLTGQ RVLGVPIIVQ ASQAEKNRVP IIVQASQAEK NR

Formula weight

16798.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 66.4 %, Completeness: 59.6 %, Completeness (bb): 63.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.6 % (1047 of 1756)	58.5 % (537 of 918)	60.1 % (408 of 679)	64.2 % (102 of 159)
Backbone	63.4 % (569 of 898)	63.8 % (196 of 307)	62.6 % (279 of 446)	64.8 % (94 of 145)
Sidechain	57.2 % (572 of 1000)	55.8 % (341 of 611)	59.5 % (223 of 375)	57.1 % (8 of 14)
Aromatic	67.1 % (47 of 70)	71.4 % (25 of 35)	62.9 % (22 of 35)
Methyl	59.0 % (118 of 200)	60.0 % (60 of 100)	58.0 % (58 of 100)

1. entity

GHMNLTPEER DARTVFCMQL AARIRPRDLE EFFSTVGKVR DVRMISDRNS RRSKGIAYVE FVDVSSVPLA IGLTGQRVLG VPIIVQASQA EKNRRRSKGI AYVEFVDVSS VPLAIGLTGQ RVLGVPIIVQ ASQAEKNRVP IIVQASQAEK NR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium acetate	[U-99% 2H]	10 mM
4	sodium azide	natural abundance	5 mM
5	DTT	natural abundance	2 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MHN, Strand ID: A Detail

Release date

2013-12-19

Citation

NMR structure of the first RRM domain of the protein RBM39 from homo sapiens
Serrano, P., Wuthrich, K., Geralt, M., Dutta, S.K.
Not known

Related entities 1. RBM39 RRM1, : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC