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BMRB: 19643

2MHO

NMR Solution State Structure of the PSD-95 PDZ1 - 5-HT2c Complex

Authors

Dorr, L.A., Phelan, M.M., Lian, L.

Assembly

5-Hydroxytryptamine Receptor 2a and 2c Variants with PSD-95 and SAP997

Entity

1. 5-Hydroxytryptamine Receptor 2a and 2c Variants with PSD-95 and SAP997, entity 1 (polymer, Thiol state: not present), 99 monomers, 10609.87 Da Detail

GAMEMEYEEI TLERGNSGLG FSIAGGTDNP HIGDDPSIFI TKIIPGGAAA QDGRLRVNDS ILFVNEVDVR EVTHSAAVEA LKEAGSIVRL YVMRRKPPA

2. 5-Hydroxytryptamine Receptor 2a and 2c Variants with PSD-95 and SAP997, entity 2 (polymer, Thiol state: not present), 9 monomers, 975.0978 Da Detail

VVSERISSV

Total weight

11584.968 Da

Max. entity weight

10609.87 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 88.9 %, Completeness: 75.3 %, Completeness (bb): 74.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.3 % (892 of 1185)	80.9 % (494 of 611)	65.5 % (305 of 466)	86.1 % (93 of 108)
Backbone	74.8 % (477 of 638)	88.7 % (197 of 222)	60.1 % (188 of 313)	89.3 % (92 of 103)
Sidechain	77.3 % (498 of 644)	76.3 % (297 of 389)	80.0 % (200 of 250)	20.0 % (1 of 5)
Aromatic	85.2 % (46 of 54)	85.2 % (23 of 27)	85.2 % (23 of 27)
Methyl	86.8 % (118 of 136)	86.8 % (59 of 68)	86.8 % (59 of 68)

1. entity 1

GAMEMEYEEI TLERGNSGLG FSIAGGTDNP HIGDDPSIFI TKIIPGGAAA QDGRLRVNDS ILFVNEVDVR EVTHSAAVEA LKEAGSIVRL YVMRRKPPA

2. entity 2

VVSERISSV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	0.5 mM
2	entity_2	natural abundance	2.5 mM
3	DTT	natural abundance	1 mM
4	sodium azide	natural abundance	0.01 w/v
5	sodium phosphate	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	[U-100% 2H]	10 %

Chem. Shift Complete2

Sequence coverage: 28.7 %, Completeness: 47.8 %, Completeness (bb): 47.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.8 % (1134 of 2370)	52.2 % (638 of 1222)	41.0 % (382 of 932)	52.8 % (114 of 216)
Backbone	47.7 % (609 of 1276)	57.4 % (255 of 444)	38.5 % (241 of 626)	54.9 % (113 of 206)
Sidechain	48.6 % (626 of 1288)	49.2 % (383 of 778)	48.4 % (242 of 500)	10.0 % (1 of 10)
Aromatic	42.6 % (46 of 108)	42.6 % (23 of 54)	42.6 % (23 of 54)
Methyl	60.7 % (165 of 272)	63.2 % (86 of 136)	58.1 % (79 of 136)

1. entity 1

GAMEMEYEEI TLERGNSGLG FSIAGGTDNP HIGDDPSIFI TKIIPGGAAA QDGRLRVNDS ILFVNEVDVR EVTHSAAVEA LKEAGSIVRL YVMRRKPPA

2. entity 2

VVSERISSV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	entity_1	[U-13C; U-15N]	0.5 mM
2	entity_2	natural abundance	2.5 mM
3	DTT	natural abundance	1 mM
4	sodium azide	natural abundance	0.01 w/v
5	sodium phosphate	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	[U-100% 2H]	10 %

Protein Blocks Logo

Calculated from 19 models in PDB: 2MHO, Strand ID: A Detail

Release date

2014-02-03

Citation

Interactions of the 5-Hydroxytryptamine Receptor 2a and 2c Variants with the PSD-MAGUK proteins, PSD-95 and SAP997
Dorr, L.A., Phelan, M.M., Lian, L.
Not known

Related entities 1. 5-Hydroxytryptamine Receptor 2a and 2c Variants with PSD-95 and SAP997, entity 1, : 3 : 245 entities Detail

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC