NMR structure of the S-linked glycopeptide sublancin 168
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS7:SG | 1:CYS36:SG |
2 | disulfide | sing | 1:CYS14:SG | 1:CYS29:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.9 % (290 of 382) | 93.4 % (183 of 196) | 45.5 % (65 of 143) | 97.7 % (42 of 43) |
Backbone | 82.9 % (184 of 222) | 100.0 % (82 of 82) | 63.1 % (65 of 103) | 100.0 % (37 of 37) |
Sidechain | 70.9 % (134 of 189) | 88.6 % (101 of 114) | 40.6 % (28 of 69) | 83.3 % (5 of 6) |
Aromatic | 30.0 % (9 of 30) | 53.3 % (8 of 15) | 0.0 % (0 of 14) | 100.0 % (1 of 1) |
Methyl | 67.6 % (23 of 34) | 100.0 % (17 of 17) | 35.3 % (6 of 17) |
1. sublancin
GLGKAQCAAL WLQCASGGTI GCGGGAVACQ NYRQFCRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | 13C,15N-labeled sublancin | [U-98% 13C; U-98% 15N] | 2 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | sublancin | natural abundance | 2 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | 15N-labeled sublancin | [U-100% 15N] | 2 mM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |