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BMRB: 19684

2MIO

Solution NMR Structure of SH3 Domain 1 of Rho GTPase-activating Protein 10 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target: HR9129A

Authors

Xu, X., Eletsky, A., Pulavarti, S.VSRK., Wang, H., O'Connell, P.T., Janjua, H., Xiao, R., Everett, J.K., Sukumaran, D.K., Montelione, G.T., Szyperski, T.

Assembly

HR9129A

Entity

1. HR9129A (polymer, Thiol state: all free), 70 monomers, 8027.964 Da Detail

MGHHHHHHSH MIRSRKARAV YPCEAEHSSE LSFEIGAIFE DVQTSREPGW LEGTLNGKRG LIPQNYVKLL

Formula weight

8027.964 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 86.2 %, Completeness (bb): 87.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (705 of 818)	87.4 % (373 of 427)	84.6 % (270 of 319)	86.1 % (62 of 72)
Backbone	87.7 % (363 of 414)	87.4 % (125 of 143)	88.7 % (181 of 204)	85.1 % (57 of 67)
Sidechain	85.3 % (399 of 468)	87.3 % (248 of 284)	81.6 % (146 of 179)	100.0 % (5 of 5)
Aromatic	52.5 % (42 of 80)	62.5 % (25 of 40)	41.0 % (16 of 39)	100.0 % (1 of 1)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. HR9129A

MGHHHHHHSH MIRSRKARAV YPCEAEHSSE LSFEIGAIFE DVQTSREPGW LEGTLNGKRG LIPQNYVKLL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.72 mM, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	HR9129A	[U-99% 13C; U-98% 15N]		0.72 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.72 mM, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	HR9129A	[U-99% 13C; U-98% 15N]		0.72 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MIO, Strand ID: A Detail

Release date

2013-12-19

Related entities 1. HR9129A, : 1 : 3 : 84 entities Detail

Interaction partners 1. HR9129A, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC