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BMRB: 19687

2MIM

immune signalling subunit

Authors

Headey, S., Berry, R., Rossjohn, J.

Assembly

immune signalling subunit

Entity

1. immune signalling subunit (polymer, Thiol state: all disulfide bound), 178 monomers, 19540.52 Da Detail

GSHMGQEEFA VEISGTTVTI TCPSSGDDIK WKPDPALGDN NKYIIQNHDS SPLTVSCTAG DQEHTMYLNA KVGSADDAKK DAAKKDDAKK DDAKKDGSVA KLGVHGLSMS VKEVSGKVFL QCQESKDLNT NYLWKKGKEE LGNMRQLDLG AIYDDPRGTY TCQRDENVNS TLHVHYRM

Formula weight

19540.52 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS22:SG	1:CYS57:SG
2	disulfide	sing	1:CYS122:SG	1:CYS162:SG

Source organism

Gallus gallus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.2 %, Completeness: 67.4 %, Completeness (bb): 72.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.4 % (1341 of 1990)	70.2 % (727 of 1036)	60.9 % (465 of 763)	78.0 % (149 of 191)
Backbone	72.5 % (767 of 1058)	81.7 % (299 of 366)	62.6 % (325 of 519)	82.7 % (143 of 173)
Sidechain	65.3 % (715 of 1095)	63.9 % (428 of 670)	69.0 % (281 of 407)	33.3 % (6 of 18)
Aromatic	60.3 % (70 of 116)	75.9 % (44 of 58)	42.9 % (24 of 56)	100.0 % (2 of 2)
Methyl	84.0 % (136 of 162)	82.7 % (67 of 81)	85.2 % (69 of 81)

1. entity

GSHMGQEEFA VEISGTTVTI TCPSSGDDIK WKPDPALGDN NKYIIQNHDS SPLTVSCTAG DQEHTMYLNA KVGSADDAKK DAAKKDDAKK DDAKKDGSVA KLGVHGLSMS VKEVSGKVFL QCQESKDLNT NYLWKKGKEE LGNMRQLDLG AIYDDPRGTY TCQRDENVNS TLHVHYRM

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.6

#	Name	Isotope labeling	Concentration
1	protein	[U-98% 13C; U-98% 15N]	0.5 mM
2	HEPES	natural abundance	50 mM
3	arginine	natural abundance	125 mM
4	glutamate	natural abundance	125 mM
5	azide	natural abundance	0.01 %
6	Roche protease inhibitor	natural abundance	0.01 %
7	EDTA	natural abundance	0.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.6

#	Name	Isotope labeling	Concentration
8	protein	[U-100% 13C; U-100% 15N; U-80% 2H]	0.5 mM
9	HEPES	natural abundance	50 mM
10	arginine	natural abundance	125 mM
11	glutamate	natural abundance	125 mM
12	azide	natural abundance	0.01 %
13	Roche protease inhibitor	natural abundance	0.01 %
14	EDTA	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MIM, Strand ID: A Detail

Release date

2014-02-09

Citation

immune signalling subunit
Berry, R., Headey, S.J., Call, M.E., McCluskey, J., Tregaskes, C., Kaufman, J., Koh, R., Mohanty, B., Scanlon, M., Call, M., Rossjohn, J.
Not known

Related entities 1. immune signalling subunit, : 1 : 5 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC