BMRB: 19692

NMR Localization of Divalent Cations at the Active Site of the Neurospora VS Ribozyme Provides Insights Into RNA-Metal Ion Interactions

Authors

Bonneau, E., Legault, P.

Assembly

Divalent Cations at the Active Site of the Neurospora VS Ribozyme

Entity

1. RNA (26-MER) (polymer, Thiol state: not present), 26 monomers, 8484.076 Da Detail

GAGCUGCAGC ACGAAAGUGA CGGCUC

2. Divalent Cations at the Active Site of the Neurospora VS Ribozyme, entity MG (non-polymer), 24.305 × 5 Da
3. Divalent Cations at the Active Site of the Neurospora VS Ribozyme, entity HOH (water), 18.015 × 9 Da

Total weight

8605.602 Da

Max. entity weight

8484.076 Da

Source organism

Neurospora crassa

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	99.0 % (406 of 410)	98.7 % (222 of 225)	100.0 % (173 of 173)	91.7 % (11 of 12)
Suger, PO4	100.0 % (286 of 286)	100.0 % (156 of 156)	100.0 % (130 of 130)
Nucleobase	96.8 % (120 of 124)	95.7 % (66 of 69)	100.0 % (43 of 43)	91.7 % (11 of 12)
Aromatic	98.2 % (108 of 110)	98.2 % (54 of 55)	100.0 % (43 of 43)	91.7 % (11 of 12)

1. RNA (26-MER)

GAGCUGCAGC ACGAAAGUGA CGGCUC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	RNA (26-MER)	[U-100% 15N]		1 mM
2	Mg2+	naturall abundance		5 mM
3	KCl	naturall abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	RNA (26-MER)	[U-100% 13C; U-100% 15N]		0.9 mM
5	Mg2+	naturall abundance		5 mM
6	KCl	naturall abundance		50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	RNA (26-MER)	phosphorothioate		0.2 ~ 0.4 mM
8	Mg2+	naturall abundance		5 mM
9	KCl	naturall abundance		50 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	RNA (26-MER)	[U-100% 13C; U-100% 15N]		0.9 mM
11	Mg2+	naturall abundance		5 mM
12	KCl	naturall abundance		50 mM

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Release date

2014-01-20

Citation

NMR localization of divalent cations at the active site of the Neurospora VS ribozyme provides insights into RNA-metal-ion interactions
Bonneau, E., Legault, P.
Biochemistry (2014), 53, 579-590, PubMed 24364590 , DOI 10.1021/bi401484a , Abstract

Related entities 1. RNA (26-MER), : 1 : 1 entities Detail

Experiments performed 5 experiments Detail