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BMRB: 19703

2MJ3

Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis

Authors

Barnwal, R., Varani, G.

Assembly

Iron-sulfur cluster binding protein from Ehrlichia chaffeensis

Entity

1. Iron-sulfur cluster binding protein from Ehrlichia chaffeensis (polymer, Thiol state: all free), 116 monomers, 12852.29 Da Detail

MPLITFISPD GSRKTYEAYD GETLLSLAHR NNVDLEGACE GSLACSTCHV IIDPSWYDIV EQHNEISDEE NDMLDLAFGL TDTSRLGCQI ILTKELDGLC VILPTETRNI SFVKNS

Formula weight

12852.29 Da

Source organism

Ehrlichia chaffeensis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.7 %, Completeness: 77.6 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.6 % (1006 of 1296)	74.7 % (496 of 664)	79.6 % (407 of 511)	85.1 % (103 of 121)
Backbone	88.2 % (607 of 688)	87.2 % (205 of 235)	88.9 % (303 of 341)	88.4 % (99 of 112)
Sidechain	69.2 % (496 of 717)	67.8 % (291 of 429)	72.0 % (201 of 279)	44.4 % (4 of 9)
Aromatic	33.3 % (26 of 78)	64.1 % (25 of 39)	0.0 % (0 of 38)	100.0 % (1 of 1)
Methyl	78.5 % (113 of 144)	75.0 % (54 of 72)	81.9 % (59 of 72)

1. Iron-sulfur cluster binding protein

MPLITFISPD GSRKTYEAYD GETLLSLAHR NNVDLEGACE GSLACSTCHV IIDPSWYDIV EQHNEISDEE NDMLDLAFGL TDTSRLGCQI ILTKELDGLC VILPTETRNI SFVKNS

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Iron-sulfur cluster binding protein	[U-95% 15N]		1.2 mM
2	Iron-sulfur cluster binding protein	[U-95% 13C; U-95% 15N]		1.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 9 models in PDB: 2MJ3, Strand ID: A Detail

Release date

2014-01-13

Citation

Backbone 1H, 13C, and 15N Chemical Shift Assignments and structure of Iron-sulfur cluster binding protein from Ehrlichia chaffeensis
Barnwal, R., Varani, G.
Not known

Related entities 1. Iron-sulfur cluster binding protein from Ehrlichia chaffeensis, : 1 : 126 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC