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BMRB: 19717

2MJD

Yah1 Oxidized

Authors

Gallo, A., Banci, L.

Assembly

Yah1 Oxidized

Entity

1. Yah1 Oxidized, entity (polymer, Thiol state: free and other bound), 115 monomers, 12624.93 Da Detail

GEELKITFIL KDGSQKTYEV CEGETILDIA QGHNLDMEGA CGGSCACSTC HVIVDPDYYD ALPEPEDDEN DMLDLAYGLT ETSRLGCQIK MSKDIDGIRV ALPQMTRNVN NNDFS

2. FE2/S2 (INORGANIC) CLUSTER (non-polymer), 175.820 Da

Total weight

12800.75 Da

Max. entity weight

12624.93 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 75.1 %, Completeness (bb): 85.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.1 % (945 of 1258)	67.2 % (437 of 650)	83.8 % (408 of 487)	82.6 % (100 of 121)
Backbone	85.2 % (581 of 682)	80.5 % (190 of 236)	88.4 % (296 of 335)	85.6 % (95 of 111)
Sidechain	67.4 % (459 of 681)	59.7 % (247 of 414)	80.5 % (207 of 257)	50.0 % (5 of 10)
Aromatic	0.0 % (0 of 60)	0.0 % (0 of 30)	0.0 % (0 of 30)
Methyl	73.7 % (87 of 118)	50.8 % (30 of 59)	96.6 % (57 of 59)

1. entity

GEELKITFIL KDGSQKTYEV CEGETILDIA QGHNLDMEGA CGGSCACSTC HVIVDPDYYD ALPEPEDDEN DMLDLAYGLT ETSRLGCQIK MSKDIDGIRV ALPQMTRNVN NNDFS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	entity	[U-13C; U-15N]	1 mM
6	potassium phosphate	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.8 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.8 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.8 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	0.8 ppm	external	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MJD, Strand ID: A Detail

Release date

2014-01-08

Citation

Functional reconstitution of mitochondrial Fe/S cluster synthesis on Isu1 reveals the involvement of ferredoxin
Webert, H., Freibert, S.A., Gallo, A., Heidenreich, T., Linne, U., Amlacher, S., Hurt, E., Muhlenhoff, U., Banci, L., Lill, R.
Nat. Commun. (2014), 5, 5013-5013, PubMed 25358379 , DOI 10.1038/ncomms6013 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19718 released on 2014-11-16
Title Yah1 reduced

Related entities 1. Yah1 Oxidized, entity, : 1 : 3 : 114 entities Detail

Interaction partners 1. Yah1 Oxidized, entity, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC