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BMRB: 19718

2MJE

Yah1 reduced

Authors

Gallo, A., Banci, L.

Assembly

Yah1 reduced

Entity

1. Yah1 reduced, entity (polymer, Thiol state: free and other bound), 115 monomers, 12624.93 Da Detail

GEELKITFIL KDGSQKTYEV CEGETILDIA QGHNLDMEGA CGGSCACSTC HVIVDPDYYD ALPEPEDDEN DMLDLAYGLT ETSRLGCQIK MSKDIDGIRV ALPQMTRNVN NNDFS

2. FE2/S2 (INORGANIC) CLUSTER (non-polymer), 175.820 Da

Total weight

12800.75 Da

Max. entity weight

12624.93 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.0 %, Completeness: 63.9 %, Completeness (bb): 73.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	63.9 % (804 of 1258)	57.4 % (373 of 650)	70.8 % (345 of 487)	71.1 % (86 of 121)
Backbone	73.2 % (499 of 682)	71.6 % (169 of 236)	74.0 % (248 of 335)	73.9 % (82 of 111)
Sidechain	57.0 % (388 of 681)	49.3 % (204 of 414)	70.0 % (180 of 257)	40.0 % (4 of 10)
Aromatic	0.0 % (0 of 60)	0.0 % (0 of 30)	0.0 % (0 of 30)
Methyl	47.5 % (56 of 118)	13.6 % (8 of 59)	81.4 % (48 of 59)

1. entity

GEELKITFIL KDGSQKTYEV CEGETILDIA QGHNLDMEGA CGGSCACSTC HVIVDPDYYD ALPEPEDDEN DMLDLAYGLT ETSRLGCQIK MSKDIDGIRV ALPQMTRNVN NNDFS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	entity	[U-13C; U-15N]	0.8 mM
7	potassium phosphate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2MJE, Strand ID: A Detail

Release date

2014-11-16

Citation

Functional reconstitution of mitochondrial Fe/S cluster synthesis on Isu1 reveals the involvement of ferredoxin
Webert, H., Freibert, S.A., Gallo, A., Heidenreich, T., Linne, U., Amlacher, S., Hurt, E., Muhlenhoff, U., Banci, L., Lill, R.
Nat. Commun. (2014), 5, 5013-5013, PubMed 25358379 , DOI 10.1038/ncomms6013 , Abstract

Related entities 1. Yah1 reduced, entity, : 1 : 3 : 114 entities Detail

Interaction partners 1. Yah1 reduced, entity, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC