BMRB: 19728

A tetrahelical DNA fold adopted by alternating GGG and GCG tracts

Authors

Kocman, V., Plavec, J.

Assembly

A tetrahelical DNA fold adopted by alternating GGG and GCG tracts

Entity

1. A tetrahelical DNA fold adopted by alternating GGG and GCG tracts (polymer, Thiol state: not present), 15 monomers, 4828.056 × 2 Da Detail

GGGAGCGAGG GAGCG

Total weight

9656.112 Da

Max. entity weight

4828.056 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.6 %, Completeness (bb): 74.3 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	80.6 % (112 of 139)	80.6 % (112 of 139)
Suger, PO4	74.3 % (78 of 105)	74.3 % (78 of 105)
Nucleobase	100.0 % (34 of 34)	100.0 % (34 of 34)
Aromatic	100.0 % (30 of 30)	100.0 % (30 of 30)

1. d(GGGAGCG)n

GGGAGCGAGG GAGCG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*GP*GP*GP*AP*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*G)-3')	natural abundance		2.8 mM
2	DNA (5'-D(*GP*GP*GP*AP*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*G)-3')	partial residue specific 15N		1 mM
3	DNA (5'-D(*GP*GP*GP*AP*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*G)-3')	residue specific 2H		1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %
6	LiCl	natural abundance		100 mM

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Release date

2014-12-21

Citation

A tetrahelical DNA fold adopted by tandem repeats of alternating GGG and GCG tracts
Kocman, V., Plavec, J.
Nat. Commun. (2014), 5, 5831-5831, PubMed 25500730 , DOI 10.1038/ncomms6831 , Abstract

Experiments performed 5 experiments Detail