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BMRB: 19735

2MJN

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1

Authors

Sattler, M., Wang, I.

Assembly

TIA-1 RRM2,3 monomer

Entity

1. TIA-1 RRM2,3 monomer (polymer, Thiol state: all free), 185 monomers, 20486.82 Da Detail

AMANHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN WATRKPPAPK STYESNTKQL SYDEVVNQSS PSNCTVYCGG VTSGLTEQLM RQTFSPFGQI MEIRVFPDKG YSFVRFNSHE SAAHAIVSVN GTTIEGHVVK CYWGK

Formula weight

20486.82 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.4 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.4 % (2031 of 2130)	96.2 % (1059 of 1101)	93.3 % (774 of 830)	99.5 % (198 of 199)
Backbone	97.7 % (1069 of 1094)	97.6 % (370 of 379)	97.2 % (523 of 538)	99.4 % (176 of 177)
Sidechain	93.9 % (1130 of 1204)	95.4 % (689 of 722)	91.1 % (419 of 460)	100.0 % (22 of 22)
Aromatic	90.3 % (213 of 236)	90.7 % (107 of 118)	89.5 % (102 of 114)	100.0 % (4 of 4)
Methyl	98.8 % (168 of 170)	97.6 % (83 of 85)	100.0 % (85 of 85)

1. TIA-1 RRM2,3

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	TIA-1 RRM2,3	[U-99% 13C; U-99% 15N]	0.1-0.7 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	10 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	TIA-1 RRM2,3	[U-99% 15N]	0.1-0.55 mM
9	sodium phosphate	natural abundance	10 mM
10	sodium chloride	natural abundance	50 mM
11	DTT	natural abundance	10 mM
12	sodium azide	natural abundance	0.02 %
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
15	TIA-1 RRM2,3	[U-10% 13C]	0.1-0.5 mM
16	sodium phosphate	natural abundance	10 mM
17	sodium chloride	natural abundance	50 mM
18	DTT	natural abundance	10 mM
19	sodium azide	natural abundance	0.02 %
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MJN, Strand ID: A Detail

Release date

2014-01-12

Citation

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1
Wang, I., Hennig, J., Jagtap, P., Sonntag, M., Valcarcel, J., Sattler, M.
Nucleic Acids Res. (2014), 42, 5949-5966, PubMed 24682828 , DOI 10.1093/nar/gku193 , Abstract

Related entities 1. TIA-1 RRM2,3 monomer, : 1 : 502 entities Detail

Interaction partners 1. TIA-1 RRM2,3 monomer, : 46 interactors Detail

More details for 46 interactors identified by 17 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC