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BMRB: 19737

2MJU

Solution structure of a C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB)

Authors

Esteve, V., Blatter, M., Allain, F.H.T.

Assembly

C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB)

Entity

1. C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB) (polymer, Thiol state: not present), 207 monomers, 22889.93 Da Detail

GRVGMPGVSA GGNTVLLVSN LNEEMVTPQS LFTLFGVYGD VQRVKILYNK KDSALIQMAD GNQSQLAMNH LNGQKMYGKI IRVTLSKHQT VQLPREGLDD QGLTKDFGNS PLHRFKKPGS KNFQNIFPPS ATLHLSNIPP SVAEEDLRTL FANTGGTVKA FKFFQDHKMA LLQMATVEEA IQALIDLHNY NLGENHHLRV SFSKSTI

Formula weight

22889.93 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 69.9 %, Completeness (bb): 76.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.9 % (1697 of 2429)	74.7 % (944 of 1264)	58.6 % (550 of 939)	89.8 % (203 of 226)
Backbone	76.1 % (932 of 1224)	87.7 % (370 of 422)	63.2 % (382 of 604)	90.9 % (180 of 198)
Sidechain	66.2 % (924 of 1395)	68.2 % (574 of 842)	62.3 % (327 of 525)	82.1 % (23 of 28)
Aromatic	28.7 % (50 of 174)	51.7 % (45 of 87)	5.7 % (5 of 87)
Methyl	89.0 % (210 of 236)	88.1 % (104 of 118)	89.8 % (106 of 118)

1. nPTB

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	nPTB	[U-100% 13C; U-100% 15N]	1 mM
2	sodium chloride	natural abundance	20 mM
3	sodium phosphate	natural abundance	10 mM
4	H2O	natural abundance	90 %
5	D2O	[U-100% 2H]	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	nPTB	[U-100% 15N]	1 mM
7	sodium chloride	natural abundance	20 mM
8	sodium phosphate	natural abundance	10 mM
9	D2O	[U-100% 2H]	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MJU, Strand ID: A Detail

Release date

2014-02-03

Citation

Solution and crystal structures of a C-terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB)
Joshi, A., Esteve, V., Buckroyd, A.N., Blatter, M., Allain, F., Curry, S.
PeerJ (2014), 2, e305-e305, PubMed 24688880 , DOI 10.7717/peerj.305 , Abstract

Related entities 1. C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB), : 1 : 5 : 1 : 150 entities Detail

Interaction partners 1. C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB), : 25 interactors Detail

More details for 25 interactors identified by 12 citations

Experiments performed 9 experiments Detail

1 3D HNCA