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BMRB: 19753

2N03

Solution NMR Structure plectin repeat domain 6 (4403-4606) of Plectin from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR6354E

Authors

Pulavarti, S., Eletsky, A., Huang, Y.J., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

Plectin repeat domain 6

Entity

1. Plectin repeat domain 6 (polymer, Thiol state: all free), 215 monomers, 23729.69 Da Detail

MGHHHHHHSH MQLASWSDPT EETGPVAGIL DTETLEKVSI TEAMHRNLVD NITGQRLLEA QACTGGIIDP STGERFPVTD AVNKGLVDKI MVDRINLAQK AFCGFEDPRT KTKMSAAQAL KKGWLYYEAG QRFLEVQYLT GGLIEPDTPG RVPLDEALQR GTVDARTAQK LRDVGAYSKY LTCPKTKLKI SYKDALDRSM VEEGTGLRLL EAAAQ

Formula weight

23729.69 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 52.8 %, Completeness (bb): 82.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.8 % (1290 of 2441)	33.0 % (418 of 1266)	70.7 % (674 of 953)	89.2 % (198 of 222)
Backbone	82.9 % (1054 of 1272)	63.6 % (279 of 439)	92.2 % (578 of 627)	95.6 % (197 of 206)
Sidechain	31.0 % (423 of 1366)	16.9 % (140 of 827)	53.9 % (282 of 523)	6.3 % (1 of 16)
Aromatic	12.5 % (18 of 144)	15.3 % (11 of 72)	8.6 % (6 of 70)	50.0 % (1 of 2)
Methyl	69.4 % (172 of 248)	58.9 % (73 of 124)	79.8 % (99 of 124)

1. Plectin

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Plectin	[U-100% 13C; U-100% 15N; U-2H; ILV-1H]	1 mM
2	MES pH 6.5	natural abundance	20 mM
3	NaCL	natural abundance	100 mM
4	CaCL2	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.02 %
7	Proteinase Inhibitors	natural abundance	1 ratio
8	DSS	natural abundance	50 uM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N03, Strand ID: A Detail

Release date

2014-02-23

Citation

Solution NMR Structure plectin repeat domain 6 (4403-4606) of Plectin from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR6354E
Pulavarti, S., Eletsky, A., Huang, Y.J., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Szyperski, T.J.
Not known

Related entities 1. Plectin repeat domain 6, : 1 : 1 : 126 entities Detail

Interaction partners 1. Plectin repeat domain 6, : 73 interactors Detail

More details for 73 interactors identified by 27 citations