BMRBj - BMREntryMaster

Found 1 Document (1.967 seconds)

BMRB: 19758

6FD7

1H, 15N and 13C resonance assignments of the two TPR domains of the human RPAP3 protein

Authors

Chagot, M., Jacquemin, C., Charpentier, B., Manival, X., Quinternet, M.

Assembly

RPAP3

Entity

1. RPAP3 (polymer, Thiol state: all free), 127 monomers, 14467.22 Da Detail

GPHMALVLKE KGNKYFKQGK YDEAIDCYTK GMDADPYNPV LPTNRASAYF RLKKFAVAES DCNLAVALNR SYTKAYSRRG AARFALQKLE EAKKDYERVL ELEPNNFEAT NELRKISQAL ASKENSY

Formula weight

14467.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.9 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.9 % (1431 of 1508)	95.5 % (757 of 793)	92.9 % (540 of 581)	100.0 % (134 of 134)
Backbone	99.1 % (745 of 752)	98.4 % (250 of 254)	99.2 % (373 of 376)	100.0 % (122 of 122)
Sidechain	92.0 % (808 of 878)	94.1 % (507 of 539)	88.4 % (289 of 327)	100.0 % (12 of 12)
Aromatic	57.1 % (72 of 126)	71.4 % (45 of 63)	42.9 % (27 of 63)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. rpap3(133-255)

GPHMALVLKE KGNKYFKQGK YDEAIDCYTK GMDADPYNPV LPTNRASAYF RLKKFAVAES DCNLAVALNR SYTKAYSRRG AARFALQKLE EAKKDYERVL ELEPNNFEAT NELRKISQAL ASKENSY

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	rpap3(133-255)	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium chloride	natural abundance	150 mM
3	DTT	[U-99% 2H]	10 mM
4	H2O	natural abundance	95 %
5	D2O	[U-100% 2H]	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Release date

2014-01-29

Citation

(1)H, (15)N and (13)C resonance assignments of the two TPR domains from the human RPAP3 protein
Chagot, M., Jacquemin, C., Branlant, C., Charpentier, B., Manival, X., Quinternet, M.
Biomol. NMR Assign. (2015), 9, 99-102, PubMed 24668569 , DOI 10.1007/s12104-014-9552-4 , Abstract

Related entities 1. RPAP3, : 1 : 4 : 312 entities Detail

Interaction partners 1. RPAP3, : 62 interactors Detail

More details for 62 interactors identified by 22 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC