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BMRB: 19771

2MKE

Solution structure of CPEB1 ZZ domain in the free state

Authors

Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.

Assembly

CPEB1ZZ

Entity

1. CPEB1ZZ (polymer, Thiol state: all other bound), 61 monomers, 7374.308 Da Detail

IDPYLEDSLC HICSSQPGPF FCRDQVCFKY FCRSCWHWRH SMEGLRHHSP LMRNQKNRDS S

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

7505.126 Da

Max. entity weight

7374.308 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 72.3 %, Completeness (bb): 72.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.3 % (534 of 739)	77.5 % (303 of 391)	60.9 % (173 of 284)	90.6 % (58 of 64)
Backbone	72.6 % (260 of 358)	88.3 % (106 of 120)	55.8 % (101 of 181)	93.0 % (53 of 57)
Sidechain	74.1 % (326 of 440)	73.4 % (199 of 271)	75.3 % (122 of 162)	71.4 % (5 of 7)
Aromatic	69.0 % (69 of 100)	68.0 % (34 of 50)	68.8 % (33 of 48)	100.0 % (2 of 2)
Methyl	92.9 % (26 of 28)	92.9 % (13 of 14)	92.9 % (13 of 14)

1. CPEB1ZZ

IDPYLEDSLC HICSSQPGPF FCRDQVCFKY FCRSCWHWRH SMEGLRHHSP LMRNQKNRDS S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CPEB1ZZ	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	sodium phosphate	natural abundance	50 mM
5	NaCl	natural abundance	100 mM
6	Arg	natural abundance	50 mM
7	Glu	natural abundance	50 mM
8	DTT	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	CPEB1ZZ	[U-100% 15N]	0.4 ~ 0.6 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %
12	sodium phosphate	natural abundance	50 mM
13	NaCl	natural abundance	100 mM
14	Arg	natural abundance	50 mM
15	Glu	natural abundance	50 mM
16	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.7 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.7 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 26 models in PDB: 2MKE, Strand ID: A Detail

Release date

2014-07-20

Citation

A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins
Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.
Genes. Dev. (2014), 28, 1498-1514, PubMed 24990967 , DOI 10.1101/gad.241133.114 , Abstract

Related entities 1. CPEB1ZZ, : 1 : 4 : 4 : 42 entities Detail

Interaction partners 1. CPEB1ZZ, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC