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BMRB: 19773

2MKF

Backbone chemical shifts for the E81 deletion mutant from RAP80 tandem UIMs

Authors

Anamika, A., Markin, C.J., Rout, M.K., Spyracopoulos, L.

Assembly

E81 deletion mutant from RAP80 tandem UIMs

Entity

1. E81 deletion mutant from RAP80 tandem UIMs (polymer, Thiol state: all free), 62 monomers, 6881.516 Da Detail

GPLGSRKIAQ MTEEQFALAL KMSEQEAREV NSQEEEEEEL LRKAIAESLN SCRPSDASAT RS

Formula weight

6881.516 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 42.4 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.4 % (294 of 693)	32.4 % (119 of 367)	44.6 % (116 of 260)	89.4 % (59 of 66)
Backbone	78.3 % (288 of 368)	92.7 % (115 of 124)	62.0 % (114 of 184)	98.3 % (59 of 60)
Sidechain	15.8 % (61 of 385)	1.6 % (4 of 243)	41.9 % (57 of 136)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 5)	0.0 % (0 of 5)
Methyl	21.4 % (12 of 56)	7.1 % (2 of 28)	35.7 % (10 of 28)

1. entity

GPLGSRKIAQ MTEEQFALAL KMSEQEAREV NSQEEEEEEL LRKAIAESLN SCRPSDASAT RS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.3, Details 50 mM TRIS, 150 mM NaCl, 2 mM DTT, pH 7.3

#	Name	Isotope labeling	Concentration
1	deltaE81-RAP80-tUIM	[U-100% 15N]	0.5 ~ 1.0 mM
2	TRIS	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.3, Details 50 mM TRIS, 150 mM NaCl, 2 mM DTT, pH 7.3

#	Name	Isotope labeling	Concentration
7	deltaE81-RAP80-tUIM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
8	TRIS	natural abundance	50 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	2 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MKF, Strand ID: A Detail

Release date

2014-02-05

Citation

Molecular basis for impaired DNA damage response function associated with the RAP80 ΔE81 defect
Anamika, A., Markin, C.J., Rout, M.K., Spyracopoulos, L.
J. Biol. Chem. (2014), 289, 12852-12862, PubMed 24627472 , DOI 10.1074/jbc.M113.538280 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19774 released on 2014-02-05
Title Backbone chemical shifts for the tandem UIMs of wild-type RAP80.

Related entities 1. E81 deletion mutant from RAP80 tandem UIMs, : 1 : 9 entities Detail

Interaction partners 1. E81 deletion mutant from RAP80 tandem UIMs, : 25 interactors Detail

More details for 25 interactors identified by 14 citations

Experiments performed 5 experiments Detail

1 2D 1H-15N HSQC