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BMRB: 19775

2MKH

Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in free state

Authors

Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.

Assembly

CPEB1RRM12

Entity

1. CPEB1RRM12 (polymer, Thiol state: all free), 217 monomers, 24343.72 Da Detail

MTWSGQLPPR NYKNPIYSCK VFLGGVPWDI TEAGLVNTFR VFGSLSVEWP GKDGKHPRCP PKGYVYLVFE LEKSVRSLLQ ACSHDPLSPD GLSEYYFKMS SRRMRCKEVQ VIPWVLADSN FVRSPSQRLD PSRTVFVGAL HGMLNAEALA AILNDLFGGV VYAGIDTDKH KYPIGSGRVT FNNQRSYLKA VSAAFVEIKT TKFTKKVQID PYLEDSL

Formula weight

24343.72 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 74.1 %, Completeness (bb): 73.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.1 % (1903 of 2569)	79.3 % (1060 of 1336)	62.2 % (630 of 1013)	96.8 % (213 of 220)
Backbone	73.3 % (933 of 1272)	85.5 % (372 of 435)	57.5 % (365 of 635)	97.0 % (196 of 202)
Sidechain	76.2 % (1142 of 1498)	76.4 % (688 of 901)	75.5 % (437 of 579)	94.4 % (17 of 18)
Aromatic	73.6 % (187 of 254)	88.2 % (112 of 127)	57.7 % (71 of 123)	100.0 % (4 of 4)
Methyl	91.5 % (214 of 234)	90.6 % (106 of 117)	92.3 % (108 of 117)

1. CPEB1RRM12

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CPEB1RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	DTT	natural abundance	1 mM
4	magnesium sulphate	natural abundance	1 mM
5	sodium phosphate	natural abundance	50 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CPEB1RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	magnesium sulphate	natural abundance	1 mM
12	sodium phosphate	natural abundance	50 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
15	CPEB1RRM12	[U-100% 13C; U-100% 15N; U-100% 2H]	0.4 ~ 0.6 mM
16	sodium chloride	natural abundance	100 mM
17	DTT	natural abundance	1 mM
18	magnesium sulphate	natural abundance	1 mM
19	sodium phosphate	natural abundance	50 mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.604 ppm	internal	direct	1.0

Referencing offset: 3.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.604 ppm	internal	direct	1.0

Referencing offset: 3.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.604 ppm	internal	direct	1.0

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MKH, Strand ID: A Detail

Release date

2014-07-20

Citation

A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins
Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.
Genes. Dev. (2014), 28, 1498-1514, PubMed 24990967 , DOI 10.1101/gad.241133.114 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 19771 released on 2014-07-20
    Title Solution structure of CPEB1 ZZ domain in the free state
  BMRB: 19776 released on 2014-07-20
    Title Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in complex with RNA
  BMRB: 19777 released on 2014-07-20
    Title Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in free state
  BMRB: 19778 released on 2014-07-20
    Title Structural model of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in complex with RNA

Related entities 1. CPEB1RRM12, : 1 : 1 : 77 entities Detail

Interaction partners 1. CPEB1RRM12, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC