BMRBj - BMREntryMaster

Found 1 Document (2.817 seconds)

BMRB: 19777

2MKJ

Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in free state

Authors

Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.

Assembly

CPEB4RRM12 in free state

Entity

1. CPEB4RRM12 in free state (polymer, Thiol state: all free), 203 monomers, 22797.72 Da Detail

SHQNGERVER YSRKVFVGGL PPDIDEDEIT ASFRRFGPLI VDWPHKAESK SYFPPKGYAF LLFQDESSVQ ALIDACIEED GKLYLCVSSP TIKDKPVQIR PWNLSDSDFV MDGSQPLDPR KTIFVGGVPR PLRAVELAMI MDRLYGGVCY AGIDTDPELK YPKGAGRVAF SNQQSYIAAI SARFVQLQHG EIDKRVEVKP YVL

Formula weight

22797.72 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.1 %, Completeness: 66.8 %, Completeness (bb): 68.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.8 % (1596 of 2389)	73.1 % (913 of 1249)	53.1 % (499 of 939)	91.5 % (184 of 201)
Backbone	68.2 % (809 of 1186)	85.4 % (346 of 405)	48.8 % (290 of 594)	92.5 % (173 of 187)
Sidechain	66.0 % (918 of 1391)	67.2 % (567 of 844)	63.8 % (340 of 533)	78.6 % (11 of 14)
Aromatic	70.2 % (146 of 208)	85.6 % (89 of 104)	53.9 % (55 of 102)	100.0 % (2 of 2)
Methyl	88.3 % (189 of 214)	89.7 % (96 of 107)	86.9 % (93 of 107)

1. CPEB4RRM12

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
14	sodium chloride	natural abundance	100 mM
15	sodium phosphate	natural abundance	50 mM
16	DTT	natural abundance	1 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
19	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
20	sodium chloride	natural abundance	100 mM
21	sodium phosphate	natural abundance	50 mM
22	DTT	natural abundance	1 mM
23	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.6 ppm	internal	direct	1.0

Referencing offset: 3.11 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.6 ppm	internal	direct	1.0

Referencing offset: 3.11 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.6 ppm	internal	direct	1.0

Referencing offset: 0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MKJ, Strand ID: A Detail

Release date

2014-07-20

Citation

A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins
Afroz, T., Skrisovska, L., Belloc, E., Boixet, J.G., Mendez, R., Allain, F.H.-T.
Genes. Dev. (2014), 28, 1498-1514, PubMed 24990967 , DOI 10.1101/gad.241133.114 , Abstract

Related entities 1. CPEB4RRM12 in free state, : 1 : 3 : 7 : 124 entities Detail

Interaction partners 1. CPEB4RRM12 in free state, : 8 interactors Detail

More details for 8 interactors identified by 6 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
14	sodium chloride	natural abundance	100 mM
15	sodium phosphate	natural abundance	50 mM
16	DTT	natural abundance	1 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

2 2D TROSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
14	sodium chloride	natural abundance	100 mM
15	sodium phosphate	natural abundance	50 mM
16	DTT	natural abundance	1 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

3 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 2D 1H-1H TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
19	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
20	sodium chloride	natural abundance	100 mM
21	sodium phosphate	natural abundance	50 mM
22	DTT	natural abundance	1 mM
23	D2O	natural abundance	100 %

7 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
19	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
20	sodium chloride	natural abundance	100 mM
21	sodium phosphate	natural abundance	50 mM
22	DTT	natural abundance	1 mM
23	D2O	natural abundance	100 %

8 3D CBCA(CO)NH

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

9 3D trHNCACB

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

10 3D trHNCA

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

11 3D trHN(CO)CA

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

12 3D trHNCO

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	CPEB4RRM12	[U-100% 15N]	0.4 ~ 0.6 mM
14	sodium chloride	natural abundance	100 mM
15	sodium phosphate	natural abundance	50 mM
16	DTT	natural abundance	1 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

13 3D trHCACO

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

14 3D hCCH-TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

15 3D HCcH-TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

16 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB4RRM12	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 ~ 0.6 mM
8	sodium chloride	natural abundance	100 mM
9	sodium phosphate	natural abundance	50 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

17 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

18 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB4RRM12	[U-100% 13C; U-100% 15N]	0.4 ~ 0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

nullKeywords CPEB4, Cytoplasmic polyadenylation, RNA recognition motif (RRM), Translational regulation

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Found 1 Document (2.817 seconds)

BMRB: 19777

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CPEB4RRM12 in free state, : 1 : 3 : 7 : 124 entities Detail

Interaction partners 1. CPEB4RRM12 in free state, : 8 interactors Detail

Experiments performed 18 experiments Detail

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