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BMRB: 19791

2MKR

Structural Characterization of a Complex Between the Acidic Transactivation Domain of EBNA2 and the Tfb1/p62 subunit of TFIIH.

Authors

R. Chabot, P., Raiola, L., Lussier-Price, M., Morse, T., Arseneault, G., Archambault, J., Omichinski, J.

Assembly

Complex Between the Acidic Transactivation Domain of EBNA2 and the Tfb1/p62 subunit of TFIIH

Entity

1. Tfb1 (polymer, Thiol state: not present), 115 monomers, 12883.54 Da Detail

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. EBNA2 (polymer, Thiol state: not present), 13 monomers, 1633.664 Da Detail

DLDESWDYIF ETT

Total weight

14517.204 Da

Max. entity weight

12883.54 Da

Source organism

Saccharomyces cerevisiae , Human gammaherpesvirus 4

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 89.1 %, Completeness: 77.3 %, Completeness (bb): 74.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.3 % (1153 of 1492)	83.8 % (652 of 778)	67.5 % (391 of 579)	81.5 % (110 of 135)
Backbone	74.4 % (564 of 758)	87.9 % (225 of 256)	60.9 % (231 of 379)	87.8 % (108 of 123)
Sidechain	81.4 % (698 of 857)	81.8 % (427 of 522)	83.3 % (269 of 323)	16.7 % (2 of 12)
Aromatic	32.6 % (30 of 92)	32.6 % (15 of 46)	34.1 % (15 of 44)	0.0 % (0 of 2)
Methyl	91.2 % (124 of 136)	91.2 % (62 of 68)	91.2 % (62 of 68)

1. Tfb1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. EBNA2

DLDESWDYIF ETT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Tfb1	[U-15N]	0.7 mM
8	EBNA2	natural abundance	2.1 mM
9	sodium phosphate	natural abundance	20 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Tfb1	[U-13C; U-15N]	0.7 mM
14	EBNA2	natural abundance	2.1 mM
15	sodium phosphate	natural abundance	20 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	EBNA2	[U-100% 15N]	0.5 mM
25	Tfb1	natural abundance	1.5 mM
26	sodium phosphate	natural abundance	20 mM
27	DTT	natural abundance	1 mM
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

Chem. Shift Complete2

Sequence coverage: 30.5 %, Completeness: 50.2 %, Completeness (bb): 49.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.2 % (1499 of 2984)	53.1 % (826 of 1556)	45.3 % (524 of 1158)	55.2 % (149 of 270)
Backbone	49.9 % (756 of 1516)	56.4 % (289 of 512)	42.5 % (322 of 758)	58.9 % (145 of 246)
Sidechain	51.5 % (882 of 1714)	51.4 % (537 of 1044)	52.8 % (341 of 646)	16.7 % (4 of 24)
Aromatic	32.6 % (60 of 184)	32.6 % (30 of 92)	33.0 % (29 of 88)	25.0 % (1 of 4)
Methyl	54.8 % (149 of 272)	54.4 % (74 of 136)	55.1 % (75 of 136)

1. Tfb1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. EBNA2

DLDESWDYIF ETT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Tfb1	[U-15N]	0.7 mM
8	EBNA2	natural abundance	2.1 mM
9	sodium phosphate	natural abundance	20 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Tfb1	[U-13C; U-15N]	0.7 mM
14	EBNA2	natural abundance	2.1 mM
15	sodium phosphate	natural abundance	20 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	EBNA2	[U-100% 15N]	0.5 mM
25	Tfb1	natural abundance	1.5 mM
26	sodium phosphate	natural abundance	20 mM
27	DTT	natural abundance	1 mM
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MKR, Strand ID: A, B Detail

Release date

2014-03-02

Citation

Structural and functional characterization of a complex between the acidic transactivation domain of EBNA2 and the Tfb1/p62 subunit of TFIIH
Chabot, P.R., Raiola, L., Lussier-Price, M., Morse, T., Arseneault, G., Archambault, J., Omichinski, J.G.
PLoS Pathog. (2014), 10, e1004042-e1004042, PubMed 24675874 , DOI 10.1371/journal.ppat.1004042 , Abstract

Related entities 1. Tfb1, : 2 : 4 entities Detail

Related entities 2. EBNA2, : 1 : 2 : 1 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

More details for 49 interactors identified by 12 citations

Interaction partners 2. EBNA2, : 13 interactors Detail

More details for 13 interactors identified by 2 citations

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

3 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Tfb1	[U-13C; U-15N]	0.7 mM
14	EBNA2	natural abundance	2.1 mM
15	sodium phosphate	natural abundance	20 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

4 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

5 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Tfb1	[U-15N]	0.7 mM
8	EBNA2	natural abundance	2.1 mM
9	sodium phosphate	natural abundance	20 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	EBNA2	[U-100% 15N]	0.5 mM
25	Tfb1	natural abundance	1.5 mM
26	sodium phosphate	natural abundance	20 mM
27	DTT	natural abundance	1 mM
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

7 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Tfb1	[U-13C; U-15N]	0.7 mM
14	EBNA2	natural abundance	2.1 mM
15	sodium phosphate	natural abundance	20 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

8 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

9 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

10 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

11 3D 1H-13C-12C intermolecular NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	Tfb1	[U-13C; U-15N]	0.7 mM
14	EBNA2	natural abundance	2.1 mM
15	sodium phosphate	natural abundance	20 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

12 3D 1H-13C-12C intermolecular NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
30	EBNA2	[U-13C; U-15N]	0.5 mM
31	Tfb1	natural abundance	1.5 mM
32	sodium phosphate	natural abundance	20 mM
33	DTT	natural abundance	1 mM
34	D2O	natural abundance	100 %

13 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

14 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

15 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

16 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

17 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

18 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

19 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

20 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

21 3D HCCH-COSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-13C; U-15N]	0.7 mM
2	EBNA2	natural abundance	2.1 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

22 3D HCCH-COSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	EBNA2	[U-13C; U-15N]	0.5 mM
19	Tfb1	natural abundance	1.5 mM
20	sodium phosphate	natural abundance	20 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_19791_2mkr.nef
Input source #2: Coordindates	2mkr.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
PSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEKMMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEKMMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNI

-------110-----
KMTLQQIISRYKDAD
|||||||||||||||
KMTLQQIISRYKDAD

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-----460-----
DLDESWDYIFETT
|||||||||||||
DLDESWDYIFETT
--------10---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	115	0	0	100.0
B	B	13	0	0	100.0

Content subtype: combined_19791_2mkr.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1134		98.3 (chain: A, length: 115)
2	Assigned chemical shifts	assigned_chem_shift_list_2	Warning	152		100.0 (chain: B, length: 13)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	2724 (1)	noe	98.3 (chain: A, length: 115), 100.0 (chain: B, length: 13)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	20 (1)	hbond	15.7 (chain: A, length: 115)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	144 (144)	.	64.3 (chain: A, length: 115), 84.6 (chain: B, length: 13)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 115, Sequence coverage: 98.3%
- Total number of assignments
  - ¹H chemical shifts: 648
  - ¹³C chemical shifts: 378
  - ¹⁵N chemical shifts: 108
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 13, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 76
  - ¹³C chemical shifts: 63
  - ¹⁵N chemical shifts: 13
- Completeness of assignments
  - Entity_assemble_ID: B
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: B

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
87	HIS	HD1	6.659

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	701	647	92.3
¹³C chemical shifts	516	378	73.3
¹⁵N chemical shifts	127	108	85.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	230	225	97.8
¹³C chemical shifts	230	113	49.1
¹⁵N chemical shifts	110	107	97.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	471	422	89.6
¹³C chemical shifts	286	265	92.7
¹⁵N chemical shifts	17	1	5.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	67	100.0
¹³C chemical shifts	67	65	97.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	15	48.4
¹³C chemical shifts	30	15	50.0
¹⁵N chemical shifts	1	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	77	75	97.4
¹³C chemical shifts	63	63	100.0
¹⁵N chemical shifts	14	13	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	25	96.2
¹³C chemical shifts	26	26	100.0
¹⁵N chemical shifts	13	13	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	50	98.0
¹³C chemical shifts	37	37	100.0
¹⁵N chemical shifts	1	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	6	100.0
¹³C chemical shifts	6	6	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	15	14	93.3
¹³C chemical shifts	14	14	100.0
¹⁵N chemical shifts	1	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 115, Sequence coverage: 98.3%
  - Entity_assembly_ID: B, Chain length: 13, Sequence coverage: 100.0%
- Total number of restraints: 2708
- Weight of restraints: 1.0 (2708)
- Potential type of restraints: square-well-parabolic (2708)
- Range of distance target values: 2.3, 5.23 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 115, Sequence coverage: 15.7%
  - Total number of restraints: 20
  - Weight of restraints: 1.0 (20)
  - Potential type of restraints: square-well-parabolic (20)
  - Range of distance target values: 1.9, 2.9 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
      None
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 115, Sequence coverage: 64.3%
  - Entity_assembly_ID: B, Chain length: 13, Sequence coverage: 84.6%
- Total number of restraints: 144
- Total number of restraints per polymer type: 144
- Weight of restraints: 1.0 (144)
- Potential type of restraints: square-well-parabolic (144)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords EBNA2, Protein, Tfb1

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Found 1 Document (1.209 seconds)

BMRB: 19791

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Related entities 1. Tfb1, : 2 : 4 entities Detail

Related entities 2. EBNA2, : 1 : 2 : 1 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

Interaction partners 2. EBNA2, : 13 interactors Detail

Experiments performed 22 experiments Detail

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