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BMRB: 19801

2MKZ

solution structure of a protein C-terminal domain

Authors

Feng, Y., Jiao, L.

Assembly

monomer1

Entity

1. monomer1 (polymer, Thiol state: all free), 141 monomers, 14814.44 Da Detail

SNAILATMNV PAGPAGGQQV DLASVLTPEI MAPILANADV QERLLPYLPS GESLPQTADE IQNTLTSPQF QQALGMFSAA LASGQLGPLM CQFGLPAEAV EAANKGDVEA FAKAMQNNAK PEQKEGDTKD KKDEEEDMSL D

Formula weight

14814.44 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 97.6 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.6 % (1509 of 1546)	97.3 % (782 of 804)	98.0 % (581 of 593)	98.0 % (146 of 149)
Backbone	97.9 % (807 of 824)	97.2 % (273 of 281)	98.5 % (407 of 413)	97.7 % (127 of 130)
Sidechain	97.4 % (831 of 853)	97.3 % (509 of 523)	97.4 % (303 of 311)	100.0 % (19 of 19)
Aromatic	95.8 % (46 of 48)	95.8 % (23 of 24)	95.8 % (23 of 24)
Methyl	98.7 % (152 of 154)	98.7 % (76 of 77)	98.7 % (76 of 77)

1. entity

SNAILATMNV PAGPAGGQQV DLASVLTPEI MAPILANADV QERLLPYLPS GESLPQTADE IQNTLTSPQF QQALGMFSAA LASGQLGPLM CQFGLPAEAV EAANKGDVEA FAKAMQNNAK PEQKEGDTKD KKDEEEDMSL D

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	40 mM
3	potassium chloride	natural abundance	20 mM
4	DSS	natural abundance	0.02 %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MKZ, Strand ID: A Detail

Release date

2015-01-04

Citation

Mechanism of the Rpn13-induced activation of Uch37
Jiao, L., Ouyang, S., Shaw, N., Song, G., Feng, Y., Niu, F., Qiu, W., Zhu, H., Hung, L., Zuo, X., Shtykova, V., Zhu, P., Dong, Y., Xu, R., Liu, Z.
Protein. Cell (2014), 5, 616-630, PubMed 24752541 , DOI 10.1007/s13238-014-0046-z , Abstract

Related entities 1. monomer1, : 1 : 25 entities Detail

Interaction partners 1. monomer1, : 47 interactors Detail

More details for 47 interactors identified by 20 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC