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BMRB: 19806

2ML5

NMR structure of hypothetical protein ZP_02064002.1 from Bacteroides ovatus ATCC 8483

Authors

Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

hypothetical protein ZP 02064002.1 from Bacteroides ovatus ATCC 8483

Entity

1. hypothetical protein ZP 02064002.1 from Bacteroides ovatus ATCC 8483 (polymer, Thiol state: all free), 155 monomers, 17942.73 Da Detail

GDSELTTQDG EDFKSFLDKF TSSAAFQYTR VKFPLKTPIT LLADDGETEK TFPFTKEKWP LLDSETMKEE RITQEEGGIY VSKFTLNEPK HKIFEAGYEE SEVDLRVEFE LQADGKWYVV DCYTGWYGYD LPIGELKQTI QNVKEENAAF KEIHP

Formula weight

17942.73 Da

Source organism

Bacteroides ovatus ATCC 8483

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 85.3 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (1594 of 1869)	92.7 % (902 of 973)	72.6 % (534 of 736)	98.8 % (158 of 160)
Backbone	83.3 % (763 of 916)	96.5 % (302 of 313)	69.2 % (315 of 455)	98.6 % (146 of 148)
Sidechain	88.1 % (967 of 1098)	90.9 % (600 of 660)	83.3 % (355 of 426)	100.0 % (12 of 12)
Aromatic	64.8 % (136 of 210)	78.1 % (82 of 105)	50.0 % (51 of 102)	100.0 % (3 of 3)
Methyl	100.0 % (146 of 146)	100.0 % (73 of 73)	100.0 % (73 of 73)

1. entity

GDSELTTQDG EDFKSFLDKF TSSAAFQYTR VKFPLKTPIT LLADDGETEK TFPFTKEKWP LLDSETMKEE RITQEEGGIY VSKFTLNEPK HKIFEAGYEE SEVDLRVEFE LQADGKWYVV DCYTGWYGYD LPIGELKQTI QNVKEENAAF KEIHP

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ML5, Strand ID: A Detail

Release date

2015-09-01

Citation

NMR structure of hypothetical protein ZP_02064002.1 from Bacteroides ovatus ATCC 8483
Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. hypothetical protein ZP 02064002.1 from Bacteroides ovatus ATCC 8483, : 1 : 2 entities Detail

Experiments performed 8 experiments Detail

1 APSY 4D-HACANH