BMRBj - BMREntryMaster

Found 1 Document (0.651 seconds)

BMRB: 19807

2ML6

NMR structure of hypothetical protein ZP_02069618.1 from Bacteroides uniformis ATCC 8492.

Authors

Wallmann, A., Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

NMR structure of hypothetical protein ZP 02069618.1 from Bacteroides uniformis ATCC 8492.

Entity

1. NMR structure of hypothetical protein ZP 02069618.1 from Bacteroides uniformis ATCC 8492. (polymer, Thiol state: all free), 148 monomers, 17295.67 Da Detail

GAEEEDFKTF LQKFTSSASF QYSRIKFPLK SPIALLKDDG ETEQTFPFTR EKWALLDEET LKEGRTTEEE GGTYISHFTV NEPAHKEFEA GYDESEPSLR VVFELTDGKW YVTDCYNDWY NFDLPINELE ETIQAVQEEN KAFEELHP

Formula weight

17295.67 Da

Source organism

Bacteroides uniformis

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.7 %, Completeness (bb): 84.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.7 % (1556 of 1774)	95.2 % (878 of 922)	75.2 % (525 of 698)	99.4 % (153 of 154)
Backbone	84.2 % (736 of 874)	96.6 % (286 of 296)	70.9 % (310 of 437)	99.3 % (140 of 141)
Sidechain	92.2 % (960 of 1041)	94.6 % (592 of 626)	88.3 % (355 of 402)	100.0 % (13 of 13)
Aromatic	67.6 % (146 of 216)	75.9 % (82 of 108)	58.1 % (61 of 105)	100.0 % (3 of 3)
Methyl	96.9 % (126 of 130)	96.9 % (63 of 65)	96.9 % (63 of 65)

1. entity

GAEEEDFKTF LQKFTSSASF QYSRIKFPLK SPIALLKDDG ETEQTFPFTR EKWALLDEET LKEGRTTEEE GGTYISHFTV NEPAHKEFEA GYDESEPSLR VVFELTDGKW YVTDCYNDWY NFDLPINELE ETIQAVQEEN KAFEELHP

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-99% 15N]	1.2 mM
2	sodium azide	natural abundance	0.03 %
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 7 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 7 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ML6, Strand ID: A Detail

Release date

2014-03-09

Citation

NMR structure of hypothetical protein ZP_02069618.1 from Bacteroides uniformis ATCC 8492
Wallmann, A., Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. NMR structure of hypothetical protein ZP 02069618.1 from Bacteroides uniformis ATCC 8492., : 1 : 4 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC