BMRB: 19821

Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx

Authors

Russo, L.

Assembly

Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx

Entity

1. Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx (polymer), 77 monomers, 9335.739 Da Detail

KAKLSLTQLI LIRLSNRGCQ TLEELEEFTQ AKREVLLVTL TRLHQRGVIY RKWRHFSGRK YREYCLKHRD ELADLEH

Formula weight

9335.739 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS19:SG	1:CYS65:SG

Source organism

Fuselloviridae

Exptl. method

solution NMR

Refine. method

MFR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 55.4 %, Completeness (bb): 69.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	55.4 % (537 of 970)	69.7 % (357 of 512)	26.9 % (101 of 375)	95.2 % (79 of 83)
Backbone	69.9 % (323 of 462)	98.1 % (154 of 157)	40.4 % (92 of 228)	100.0 % (77 of 77)
Sidechain	38.1 % (222 of 582)	57.2 % (203 of 355)	7.7 % (17 of 221)	33.3 % (2 of 6)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 35)	0.0 % (0 of 1)
Methyl	44.8 % (43 of 96)	81.3 % (39 of 48)	8.3 % (4 of 48)

1. Stf76

KAKLSLTQLI LIRLSNRGCQ TLEELEEFTQ AKREVLLVTL TRLHQRGVIY RKWRHFSGRK YREYCLKHRD ELADLEH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Stf76	[U-100% 13C; U-100% 15N]		100-200 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	D2O	natural abundance		10 %
6	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2MLG, Strand ID: A Detail

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Release date

2014-03-09

Citation

Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family
Contursi, P., Farina, B., Pirone, L., Fusco, S., Russo, L., Bartolucci, S., Fattorusso, R., Pedone, E.
Nucleic Acids Res. (2014), 42, 5993-6011, PubMed 24682827 , DOI 10.1093/nar/gku215 , Abstract

Related entities 1. Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx, : 1 : 14 entities Detail

Experiments performed 5 experiments Detail