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BMRB: 19852

2MMC

NUCLEOTIDE-FREE HUMAN RAN GTPASE

Authors

Bacot-Davis, V.R., Palmenberg, A.C.

Assembly

NUCLEOTIDE-FREE HUMAN RAN GTPASE

Entity

1. NUCLEOTIDE-FREE HUMAN RAN GTPASE (polymer, Thiol state: all free), 216 monomers, 24422.78 Da Detail

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Formula weight

24422.78 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 86.7 %, Completeness (bb): 87.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.7 % (2232 of 2575)	84.1 % (1125 of 1337)	87.2 % (882 of 1012)	99.6 % (225 of 226)
Backbone	87.0 % (1107 of 1272)	65.2 % (283 of 434)	97.9 % (621 of 634)	99.5 % (203 of 204)
Sidechain	88.0 % (1324 of 1505)	93.4 % (843 of 903)	79.1 % (459 of 580)	100.0 % (22 of 22)
Aromatic	97.9 % (229 of 234)	100.0 % (117 of 117)	95.6 % (109 of 114)	100.0 % (3 of 3)
Methyl	98.8 % (253 of 256)	98.4 % (126 of 128)	99.2 % (127 of 128)

1. entity

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2MMC, Strand ID: A Detail

Release date

2014-10-19

Citation

Nuclear Magnetic Resonance Structure of Ran GTPase Determines C-terminal Tail Conformational Dynamics
Bacot-Davis, V.R., Palmenberg, A.C.
J. Biomol. NMR

Related entities 1. NUCLEOTIDE-FREE HUMAN RAN GTPASE, : 1 : 70 : 1 : 32 : 91 entities Detail

Interaction partners 1. NUCLEOTIDE-FREE HUMAN RAN GTPASE, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

4 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

7 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

10 3D HNCACB

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

11 31P NMR

Instrument

Bruker Avance - 600 MHz Bruker AVIII-600 MHz Avance console. Dexter.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5mM [U 15N, U 13C]-Ran GTPase. Buffer: 20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Type	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]		0.5 mM
2	D2O	[U-100% 2H]		10 %
3	HEPES	natural abundance		20 mM
4	DTT	natural abundance		2 mM
5	sodium azide	natural abundance		0.04 %
6	potassium chloride	natural abundance		100 mM
7	magnesium chloride	natural abundance		2 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

nullKeywords TRANSPORT PROTEIN, G PROTEIN, NUCLEOTIDE-BINDING, GTP-BINDING, PROTEIN TRANSPORT, CYTOPLASM, NUCLEUS, EDTA