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BMRB: 19854

2MMG

Structural Characterization of the Mengovirus Leader Protein Bound to Ran GTPase by Nuclear Magnetic Resonance

Authors

Bacot-Davis, V.R., Palmenberg, A.C., Cornilescu, C.C., Markley, J.L.

Assembly

Mengovirus Leader Protein Bound to Ran GTPase

Entity

1. Mengovirus Leader Protein Bound to Ran GTPase (polymer, Thiol state: all free), 216 monomers, 24422.78 Da Detail

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Formula weight

24422.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 85.9 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.9 % (2213 of 2575)	82.6 % (1105 of 1337)	87.3 % (883 of 1012)	99.6 % (225 of 226)
Backbone	85.6 % (1089 of 1272)	62.2 % (270 of 434)	97.2 % (616 of 634)	99.5 % (203 of 204)
Sidechain	87.7 % (1320 of 1505)	92.5 % (835 of 903)	79.8 % (463 of 580)	100.0 % (22 of 22)
Aromatic	100.0 % (234 of 234)	100.0 % (117 of 117)	100.0 % (114 of 114)	100.0 % (3 of 3)
Methyl	96.9 % (248 of 256)	96.1 % (123 of 128)	97.7 % (125 of 128)

1. entity

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details RAN GTPASE TITRATED WITH UNLABELED L MENGO. BUFFER:20mM HEPES (pH 7.4), 100mM KCl (99.9% purity), 2mM MgCl2 (99.9% purity), 2mM DTT, and 0.04% NaN3.

#	Name	Isotope labeling	Concentration
1	RAN GTPASE	[U-100% 13C; U-100% 15N]	0.5 mM
2	L MENGO	natural abundance	0.5 mM
3	HEPES	natural abundance	20 mM
4	potassium chloride	natural abundance	100 mM
5	magnesium chloride	natural abundance	2 mM
6	DTT	natural abundance	2 mM
7	sodium azide	natural abundance	0.04 %
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2MMG, Strand ID: A Detail

Release date

2014-10-06

Citation

Solution structures of Mengovirus Leader protein, its phosphorylated derivatives, and in complex with nuclear transport regulatory protein, RanGTPase
Bacot-Davis, V.R., Ciomperlik, J.J., Basta, H.A., Cornilescu, C.C., Palmenberg, A.C.
Proc. Natl. Acad. Sci. U. S. A. (2014), 111, 15792-15797, PubMed 25331866 , DOI 10.1073/pnas.1411098111 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 19855 released on 2014-10-06
    Title Structural Characterization of the Mengovirus Leader Protein Bound to Ran GTPase by Nuclear Magnetic Resonance
  BMRB: 19857 released on 2014-10-06
    Title NMR Studies of the Phosphorylation of the Mengovirus Leader Protein Reveal Stabilization of Intermolecular Domain Interactions
  BMRB: 19858 released on 2014-10-06
    Title NMR Studies of the Phosphorylation of the Mengovirus Leader Protein Reveal Stabilization of Intermolecular Domain Interactions
  BMRB: 19084 released on 2013-04-16
    Title The Mengovirus Leader protein

Related entities 1. Mengovirus Leader Protein Bound to Ran GTPase, : 1 : 70 : 1 : 32 : 91 entities Detail

Interaction partners 1. Mengovirus Leader Protein Bound to Ran GTPase, : 1 interactors Detail

More details for 1 interactors identified by 1 citations