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BMRB: 19866

2ND4

NMR assignment of the amylase-binding protein A from Streptococcus Parasanguinis

Authors

Liu, B., Zhou, F., Wu, H., Matthews, S.

Assembly

AbpA

Entity

1. AbpA (polymer, Thiol state: all free), 186 monomers, 20659.28 Da Detail

SEFELRRQAC GRQGENPSAS NQLIQKKYVS WRDAADEANT QVAAHEAEIK EETLRQPGVV AAQQALDKAN AIVGHDHEQA VKRAQEDYNT AYNEAYNTVR NRYIQVLQQK YIEAAKAQGN YYDETAVEAN RTNEQRIADD IKAQTGKDVT VTKDENGNYV VKDEKGNVVA TVDKDGKTVK ADAKAG

Formula weight

20659.28 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 85.9 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.9 % (1796 of 2091)	82.8 % (902 of 1090)	91.1 % (716 of 786)	82.8 % (178 of 215)
Backbone	92.5 % (1029 of 1112)	91.3 % (347 of 380)	92.7 % (508 of 548)	94.6 % (174 of 184)
Sidechain	79.9 % (923 of 1155)	77.0 % (547 of 710)	89.9 % (372 of 414)	12.9 % (4 of 31)
Aromatic	90.6 % (96 of 106)	94.3 % (50 of 53)	86.5 % (45 of 52)	100.0 % (1 of 1)
Methyl	96.9 % (188 of 194)	96.9 % (94 of 97)	96.9 % (94 of 97)

1. AbpA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 5

#	Name	Isotope labeling	Concentration
1	H2O	natural abundance	4.8 na
2	sodium chloride	natural abundance	50 mM
3	potassium phosphate	natural abundance	50 mM
4	AbpA	[U-13C; U-15N]	0.0 ~ 0.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.8 ppm	internal	indirect	0.0
¹H	water	protons	4.8 ppm	internal	direct	1.0
¹⁵N	water	protons	4.8 ppm	internal	indirect	0.0

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.8 ppm	internal	indirect	0.0
¹H	water	protons	4.8 ppm	internal	direct	1.0
¹⁵N	water	protons	4.8 ppm	internal	indirect	0.0

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.8 ppm	internal	indirect	0.0
¹H	water	protons	4.8 ppm	internal	direct	1.0
¹⁵N	water	protons	4.8 ppm	internal	indirect	0.0

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.8 ppm	internal	indirect	0.0
¹H	water	protons	4.8 ppm	internal	direct	1.0
¹⁵N	water	protons	4.8 ppm	internal	indirect	0.0

Referencing offset: -0.18 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2ND4, Strand ID: A Detail

Release date

2014-10-07

Citation

NMR assignment of the amylase-binding protein A from Streptococcus parasanguinis
Liu, B., Zhou, F., Wu, H., Matthews, S.
Biomol. NMR Assign. (2015), 9, 173-175, PubMed 25016927 , DOI 10.1007/s12104-014-9568-9 , Abstract

Related entities 1. AbpA, : 1 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC